Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-12-9
pubmed:abstractText
Proteolytically-derived amino-terminal fragments from the Bacillus thuringiensis delta-endotoxin, CryIA(c), demonstrated ion channel activity in two in vitro assays in the absence of any membrane receptors. A 24.5 kDa fragment (from Spodoptera frugiperda digestive proteolysis) formed cation-selective channels (10mV for a 3:1 salt gradient) in planar lipid bilayers up to several hundred picoSiemen conductance. A 21.5 kDa fragment doublet (from alkaline hydrolysis) also showed large conductance in planar lipid bilayers and resulted in nearly 2-fold greater 86Rb(+)-K+ efflux from phosphatidylcholine vesicles than 55 kDa CryIA(c). In preliminary screening bioassays, however, the 21.5 kDa fragments showed no insecticidal activity toward neonate Heliothis virescens. Ion channel activity of these putative alpha-helical region fragments support this region being responsible for ion channel properties of the parent delta-endotoxin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins, http://linkedlifedata.com/resource/pubmed/chemical/Endopeptidases, http://linkedlifedata.com/resource/pubmed/chemical/Endotoxins, http://linkedlifedata.com/resource/pubmed/chemical/Hemolysin Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels, http://linkedlifedata.com/resource/pubmed/chemical/Lipid Bilayers, http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments, http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholines, http://linkedlifedata.com/resource/pubmed/chemical/Potassium, http://linkedlifedata.com/resource/pubmed/chemical/Rubidium, http://linkedlifedata.com/resource/pubmed/chemical/insecticidal crystal protein...
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
196
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
921-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Ion channel activity of N-terminal fragments from CryIA(c) delta-endotoxin.
pubmed:affiliation
Ecogen, Inc, Langhorne, PA 19047.
pubmed:publicationType
Journal Article