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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1993-12-6
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pubmed:abstractText |
The aim of the present study was to search for electrophysiological effects of human lipoproteins on membrane currents in mouse peritoneal macrophages which had been cultured for 5 to 20 days. Whole-cell currents were recorded by using a voltage-clamp technique. Low density lipoprotein (LDL, 100 micrograms/ml) increased a slowly activating nonspecific cation current (iso) in the positive potential range to 244 +/- 23% of the reference (test potential + 55 mV, n = 13, P < 0.005). Augmentation of current resulted out of a negative shift of the activation curve along the voltage axis (-22 mV) and an increase of maximally available current. Furthermore, LDL increased a rapidly activating outward current (ifo) at test potentials positive to the potassium equilibrium potential. At +55 mV ifo-amplitude increased to 165 +/- 14% of reference (n = 16, P < 0.005). LDL-induced effects on ifo-current could be mimicked by application of the calcium ionophore A 23,187 (1 mumol/l) which led to an increase of ifo-current to 161 +/- 25% of the reference (test potential +55 mV, n = 11, P < 0.005). Acetylated-LDL (100 micrograms/ml, 5-15 min) produced no significant effect on the membrane currents under investigation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0028-1298
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
348
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
207-12
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7694157-Animals,
pubmed-meshheading:7694157-Calcimycin,
pubmed-meshheading:7694157-Cells, Cultured,
pubmed-meshheading:7694157-Ion Channels,
pubmed-meshheading:7694157-Lipoproteins, LDL,
pubmed-meshheading:7694157-Macrophages,
pubmed-meshheading:7694157-Male,
pubmed-meshheading:7694157-Membrane Potentials,
pubmed-meshheading:7694157-Mice,
pubmed-meshheading:7694157-Peritoneal Cavity
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pubmed:year |
1993
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pubmed:articleTitle |
Activation of membrane outward currents by human low density lipoprotein in mouse peritoneal macrophages.
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pubmed:affiliation |
Institut für Pharmakologie, Universität Düsseldorf, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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