Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-12-3
pubmed:abstractText
The nature and function of previously described perineuronal nets are still obscure. In the present study their polyanionic components were demonstrated in the rat brain using colloidal iron hydroxide (CIH) staining. In subcortical regions, such as the red nucleus, cerebellar, and vestibular nuclei, most neurons were ensheathed by CIH-binding material. In the cerebral cortex perineuronal nets were seen around numerous nonpyramidal neurons. Biotinylated hyaluronectin revealed that hyaluronan occurs in perineuronal nets. Two plant lectins [Wisteria floribunda agglutinin (WFA) and Vicia villosa agglutinin (VVA)] with affinity for N-acetylgalactosamine visualized perineuronal nets similar to those rich in anionic components. Glutamic acid decarboxylase (GAD)-immunoreactive synaptic boutons were shown to occupy numerous meshes of perineuronal VVA-positive nets. Electron microscopically, VVA binding sites were scattered throughout perisynaptic profiles, but accumulated at membranes and in the extracellular space except not in synaptic clefts. To investigate the spatial relationship between glial cell processes and perineuronal nets, two astrocytic markers (S100-protein and glutamine synthetase) were visualized at the light and electron microscopic level. Two methods to detect microglia by the use of Griffonia simplicifolia agglutinin (GSA I-B4) and the monoclonal antibody, OX-42, were also applied. Labelled structures forming perineuronal nets were observed with both astrocytic, but not with microglial, markers. It is concluded that perineuronal nets are composed of a specialized type of glia-associated extracellular matrix rich in polyanionic groups and N-acetylgalactosamine. The net-like appearance is due to perisynaptic arrangement of the astrocytic processes and these extracellular components. Similar to the ensheathment of nodes of Ranvier, perineuronal nets may provide a special ion buffering capacity required around various, perhaps highly active, types of neurons.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Acetylgalactosamine, http://linkedlifedata.com/resource/pubmed/chemical/Anions, http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers, http://linkedlifedata.com/resource/pubmed/chemical/Colloids, http://linkedlifedata.com/resource/pubmed/chemical/Glutamate Decarboxylase, http://linkedlifedata.com/resource/pubmed/chemical/Glutamate-Ammonia Ligase, http://linkedlifedata.com/resource/pubmed/chemical/Hyaluronic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Hydroxides, http://linkedlifedata.com/resource/pubmed/chemical/Iron Compounds, http://linkedlifedata.com/resource/pubmed/chemical/Lectins, http://linkedlifedata.com/resource/pubmed/chemical/Organic Chemicals, http://linkedlifedata.com/resource/pubmed/chemical/S100 Proteins, http://linkedlifedata.com/resource/pubmed/chemical/gamma-Aminobutyric Acid
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0894-1491
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
183-200
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7693589-Acetylgalactosamine, pubmed-meshheading:7693589-Animals, pubmed-meshheading:7693589-Anions, pubmed-meshheading:7693589-Biological Markers, pubmed-meshheading:7693589-Brain, pubmed-meshheading:7693589-Brain Chemistry, pubmed-meshheading:7693589-Colloids, pubmed-meshheading:7693589-Cytoplasm, pubmed-meshheading:7693589-Extracellular Matrix, pubmed-meshheading:7693589-Glutamate Decarboxylase, pubmed-meshheading:7693589-Glutamate-Ammonia Ligase, pubmed-meshheading:7693589-Hyaluronic Acid, pubmed-meshheading:7693589-Hydroxides, pubmed-meshheading:7693589-Iron Compounds, pubmed-meshheading:7693589-Lectins, pubmed-meshheading:7693589-Microglia, pubmed-meshheading:7693589-Neuroglia, pubmed-meshheading:7693589-Neurons, pubmed-meshheading:7693589-Organic Chemicals, pubmed-meshheading:7693589-Rats, pubmed-meshheading:7693589-Rats, Sprague-Dawley, pubmed-meshheading:7693589-Rats, Wistar, pubmed-meshheading:7693589-S100 Proteins, pubmed-meshheading:7693589-Staining and Labeling, pubmed-meshheading:7693589-Synapses, pubmed-meshheading:7693589-gamma-Aminobutyric Acid
pubmed:year
1993
pubmed:articleTitle
Perineuronal nets provide a polyanionic, glia-associated form of microenvironment around certain neurons in many parts of the rat brain.
pubmed:affiliation
Paul Flechsig Institute for Brain Research, University of Leipzig, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't