Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1993-12-10
|
| pubmed:abstractText |
Cytotoxic T lymphocyte (CTL) activity for hepatitis B virus (HBV) DNA-transfected HepG2 cells (designated HB3-5), which secrete HBsAg, HBeAg and HBV particles, was investigated in 31 patients with chronic HBV infection (18 chronic hepatitis and 13 asymptomatic carriers). 51Cr-labeled HB3-5 with A2 as a major HLA class I antigen served as target cells and T cells from peripheral blood mononuclear cells as effector cells. The CTL activity was measured by a 51Cr release assay. Patients were divided into two groups, the A2 group bearing HLA-A2 and the non-A2 group not bearing HLA-A2. Chronic hepatitis patients in the A2 group showed increased HBV Ag-specific cytotoxicity compared with that seen in the non-A2 group (5.2 +/- 3.1% vs. 0.9 +/- 1.4%; means +/- SD, P < 0.01). In the A2 group with chronic hepatitis, the cytotoxicity was greater in anti-HBe positive patients than in HBeAg positive patients (8.6 +/- 1.9% vs. 3.4 +/- 2.0%, P < 0.01), and asymptomatic carriers showed less cytotoxicity (0.35 +/- 0.31%, P < 0.001) compared with chronic hepatitis patients. In the non-A2 group, HBV Ag-specific CTL activity was negligible in most patients and thus no differences were found among all patient groups. The HBV Ag-specific cytotoxicity was inhibited by antibodies to CD3, HLA class I and hepatitis B nucleocapsid antigens. Removal of CD8+ cells also resulted in marked decrease in the cytotoxicity. These findings indicate that HBV Ag-specific cytotoxicity reflects liver cell damage and HBeAg/anti-HBe status. Furthermore, our assay system appears to be useful to assess CTL response in patients with chronic HBV infection.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A2 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B Antigens
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0435-1339
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
28
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
657-65
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7693538-Adult,
pubmed-meshheading:7693538-Cytotoxicity, Immunologic,
pubmed-meshheading:7693538-DNA, Viral,
pubmed-meshheading:7693538-Epitopes,
pubmed-meshheading:7693538-Female,
pubmed-meshheading:7693538-HLA-A2 Antigen,
pubmed-meshheading:7693538-Hepatitis, Chronic,
pubmed-meshheading:7693538-Hepatitis B,
pubmed-meshheading:7693538-Hepatitis B Antibodies,
pubmed-meshheading:7693538-Hepatitis B Antigens,
pubmed-meshheading:7693538-Hepatitis B virus,
pubmed-meshheading:7693538-Hepatoblastoma,
pubmed-meshheading:7693538-Humans,
pubmed-meshheading:7693538-Liver Neoplasms,
pubmed-meshheading:7693538-Male,
pubmed-meshheading:7693538-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:7693538-Transfection,
pubmed-meshheading:7693538-Tumor Cells, Cultured
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Cytotoxic T lymphocyte activity to hepatitis B virus DNA-transfected HepG2 cells in patients with chronic hepatitis B.
|
| pubmed:affiliation |
Third Department of Internal Medicine, Nagoya University School of Medicine, Japan.
|
| pubmed:publicationType |
Journal Article
|