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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1993-12-3
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pubmed:abstractText |
Quantitative crossed immunoelectrophoresis was used to evaluate the antigenic similarity of Pseudomonas aeruginosa and Pseudomonas cepacia GroEL proteins. We found that the two proteins showed 75% identity. By using a panel of monoclonal antibodies against the P. aeruginosa GroEL protein, we identified 10 monoclonal antibodies which cross-reacted with the P. cepacia GroEL protein and 21 monoclonal antibodies which recognized type-specific epitopes on the P. aeruginosa GroEL protein. In crossed immunoelectrophoresis two different fractions of GroEL reactive material could be resolved. These fractions showed a reaction of partial identity. Examination of the two immunoprecipitates by Western blotting, showed that both fractions consisted of anti-60 kDa GroEL reactive protein. One fraction, in addition, contained LPS with a characteristic 'ladder' reaction in modified Western blotting. We therefore conclude that this fraction represents a complex between LPS and GroEL.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0903-4641
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
101
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
621-30
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7692893-Antibodies, Monoclonal,
pubmed-meshheading:7692893-Antigens, Bacterial,
pubmed-meshheading:7692893-Bacterial Proteins,
pubmed-meshheading:7692893-Blotting, Western,
pubmed-meshheading:7692893-Burkholderia cepacia,
pubmed-meshheading:7692893-Chaperonin 60,
pubmed-meshheading:7692893-Cross Reactions,
pubmed-meshheading:7692893-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7692893-Epitopes,
pubmed-meshheading:7692893-Heat-Shock Proteins,
pubmed-meshheading:7692893-Humans,
pubmed-meshheading:7692893-Immunoelectrophoresis,
pubmed-meshheading:7692893-Lipopolysaccharides,
pubmed-meshheading:7692893-Pseudomonas aeruginosa
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pubmed:year |
1993
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pubmed:articleTitle |
Antigenic analysis of Pseudomonas aeruginosa and Pseudomonas cepacia GroEL proteins and demonstration of a lipopolysaccharide-associated GroEL fraction in P. aeruginosa.
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pubmed:affiliation |
Department of Medical Microbiology, University of Copenhagen, Denmark.
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pubmed:publicationType |
Journal Article
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