Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1993-11-18
pubmed:abstractText
The human keratin 18 (K18) gene is expressed in a variety of adult simple epithelial tissues, including liver, intestine, lung, and kidney, but is not normally found in skin, muscle, heart, spleen, or most of the brain. Transgenic animals derived from the cloned K18 gene express the transgene in appropriate tissues at levels directly proportional to the copy number and independently of the sites of integration. We have investigated in transgenic mice the dependence of K18 gene expression on the distal 5' and 3' flanking sequences and upon the RNA polymerase III promoter of an Alu repetitive DNA transcription unit immediately upstream of the K18 promoter. Integration site-independent expression of tandemly duplicated K18 transgenes requires the presence of either an 825-bp fragment of the 5' flanking sequence or the 3.5-kb 3' flanking sequence. Mutation of the RNA polymerase III promoter of the Alu element within the 825-bp fragment abolishes copy number-dependent expression in kidney but does not abolish integration site-independent expression when assayed in the absence of the 3' flanking sequence of the K18 gene. The characteristics of integration site-independent expression and copy number-dependent expression are separable. In addition, the formation of the chromatin state of the K18 gene, which likely restricts the tissue-specific expression of this gene, is not dependent upon the distal flanking sequences of the 10-kb K18 gene but rather may depend on internal regulatory regions of the gene.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-1373066, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-1495984, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-1508212, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-1545826, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-1696235, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-1697294, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-1710768, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-1859421, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2158907, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2188092, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2295312, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2304463, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2304465, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2390972, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2434380, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2454868, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2467843, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2469635, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2471065, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2493384, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2540418, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2564317, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2721958, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2748594, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2797152, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2822260, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2850469, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-2924354, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-3023916, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-3545063, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-3690667, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-3736674, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-518835, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-6158890, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-6186379, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-6327068, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-7680099, http://linkedlifedata.com/resource/pubmed/commentcorrection/7692231-7681143
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6742-51
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:7692231-Animals, pubmed-meshheading:7692231-Base Sequence, pubmed-meshheading:7692231-Blotting, Northern, pubmed-meshheading:7692231-Brain, pubmed-meshheading:7692231-Cells, Cultured, pubmed-meshheading:7692231-Cloning, Molecular, pubmed-meshheading:7692231-DNA Primers, pubmed-meshheading:7692231-Endoderm, pubmed-meshheading:7692231-Exons, pubmed-meshheading:7692231-Female, pubmed-meshheading:7692231-Humans, pubmed-meshheading:7692231-Intestines, pubmed-meshheading:7692231-Keratins, pubmed-meshheading:7692231-Kidney, pubmed-meshheading:7692231-Liver, pubmed-meshheading:7692231-Mice, pubmed-meshheading:7692231-Mice, Transgenic, pubmed-meshheading:7692231-Molecular Sequence Data, pubmed-meshheading:7692231-Organ Specificity, pubmed-meshheading:7692231-Promoter Regions, Genetic, pubmed-meshheading:7692231-RNA, Messenger, pubmed-meshheading:7692231-RNA Polymerase III, pubmed-meshheading:7692231-Repetitive Sequences, Nucleic Acid, pubmed-meshheading:7692231-Restriction Mapping, pubmed-meshheading:7692231-Transcription, Genetic, pubmed-meshheading:7692231-Transfection
pubmed:year
1993
pubmed:articleTitle
Alu sequence involvement in transcriptional insulation of the keratin 18 gene in transgenic mice.
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