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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1993-11-24
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pubmed:abstractText |
Sequences encoding the transmembrane domain of the Rous sarcoma virus envelope (Env) glycoprotein were deleted and replaced with sequences that signal addition of a glycosyl phosphatidylinositol (GPI) membrane anchor. Stable NIH 3T3 cell lines expressing either the wild-type transmembrane-anchored Env or the Env chimera with a GPI tail were established. The GPI-anchored envelope glycoprotein is expressed, oligomerized, and transported to the cell surface in a manner identical to that of its wild-type transmembrane-anchored counterpart. The GPI-linked protein is quantitatively removed from the cell surface by treatment with phosphatidylinositol phospholipase C. The phosphatidylinositol phospholipase C-released, water-soluble Env glycoprotein ectodomain retains the wild-type oligomeric structure and provides a useful tool for studying the subgroup-specific binding and fusion activities of a prototypic retroviral Env glycoprotein.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-1380451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-1439803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-1629960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-1668269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-1696397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-1739972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-1829108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-1836788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-189094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-206014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-2148872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-2157795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-2168989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-2446389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-2447101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-2450679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-2555557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-2660823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-2824857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-2847170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-2981357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-3052274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-3107838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-3259178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-3304138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-3323813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-3629244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-4626449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-6330999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-6951181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-7464906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-8457561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7692089-8460475
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:geneSymbol |
env
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6889-92
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7692089-3T3 Cells,
pubmed-meshheading:7692089-Animals,
pubmed-meshheading:7692089-Antigens, CD,
pubmed-meshheading:7692089-Antigens, CD55,
pubmed-meshheading:7692089-Avian Sarcoma Viruses,
pubmed-meshheading:7692089-Glycosylphosphatidylinositols,
pubmed-meshheading:7692089-Membrane Glycoproteins,
pubmed-meshheading:7692089-Mice,
pubmed-meshheading:7692089-Phosphatidylinositol Diacylglycerol-Lyase,
pubmed-meshheading:7692089-Phosphoric Diester Hydrolases,
pubmed-meshheading:7692089-Recombinant Fusion Proteins,
pubmed-meshheading:7692089-Solubility,
pubmed-meshheading:7692089-Viral Envelope Proteins
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pubmed:year |
1993
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pubmed:articleTitle |
Generation of a water-soluble oligomeric ectodomain of the Rous sarcoma virus envelope glycoprotein.
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pubmed:affiliation |
Department of Biochemistry, School of Medicine, University of California, San Francisco 94143-0450.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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