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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1993-11-2
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pubmed:abstractText |
Enzyme-linked immunosorbent assays (ELISA) and immunoblots using either whole-cell lysates of Borrelia burgdorferi or an antigenic region of flagellin (41-G) as the antigen were performed, and the abilities of the two assays to detect antibodies to this spirochete in dog, cottontail rabbit, and horse sera were compared. Assays using whole-cell B. burgdorferi lysates as the antigen were more sensitive for detecting antibodies. ELISA with 41-G as the antigen were specific for Borrelia antibodies but were not as sensitive as the assays with whole-cell lysates coated to the solid phase. Use of recombinant full-length flagellin, rather than 41-G, as the antigen in immunoblots increased the sensitivity of each assay. However, antibodies to other bacterial antigens cross-react with whole flagellin and may account for false-positive results. Antibodies to B. burgdorferi outer surface protein A or B were usually undetected when the sera were tested by immunoblotting methods. Borrelia lysates or the 41-G antigen may be used in ELISA or immunoblots to document host exposure to this spirochete. The use of 41-G as the antigen may increase the specificity of an assay or help confirm the serologic diagnosis of Lyme borreliosis in dogs, horses, and cottontail rabbits.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-1280650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-1307866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-1370277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-1548169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-1583333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-1704884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-1894359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-1993751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-2186910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-2237407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-2345309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-2351732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-2380356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-2380361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-2668764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-2684937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-2685928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-3041881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-3190094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-3225808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-3298452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-3366860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-3531237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-3997647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-3997648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-6373966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-7043737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-8384628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7691874-8433346
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2451-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7691874-Animals,
pubmed-meshheading:7691874-Antibodies, Bacterial,
pubmed-meshheading:7691874-Borrelia burgdorferi Group,
pubmed-meshheading:7691874-Dogs,
pubmed-meshheading:7691874-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:7691874-Epitopes,
pubmed-meshheading:7691874-Flagellin,
pubmed-meshheading:7691874-Horses,
pubmed-meshheading:7691874-Immunoblotting,
pubmed-meshheading:7691874-Rabbits,
pubmed-meshheading:7691874-Recombinant Proteins
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pubmed:year |
1993
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pubmed:articleTitle |
Serologic analysis of dogs, horses, and cottontail rabbits for antibodies to an antigenic flagellar epitope of Borrelia burgdorferi.
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pubmed:affiliation |
Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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