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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-11-4
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pubmed:abstractText |
Pancreatic secretory trypsin inhibitor (PSTI) has been suggested to be an acute-phase reactant in humans and to be induced by inflammatory cytokines such as the interleukins IL-1 and IL-6. We report that PSTI is synthesized in hepatoma cells and that the gene expression is augmented by IL-6. The start points (tsp) for basal and augmented transcription are exactly the same as the tsp in normal pancreas. Analysis of the PSTI gene revealed that a 40-bp DNA fragment located between kb -3.84 and -3.80 carries the element responsible for both transcriptional activity and IL-6-induced gene expression. This 40-bp fragment contains TTGNNGNAATG, the consensus sequence for the NF-IL6-binding site, which is also known as the IL-6-responsive element that is conserved among various acute-phase genes. The basal activity was augmented by another sequence that lies between kb -4.0 and -3.9.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
131
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pubmed:geneSymbol |
IL6-RE
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
275-80
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:7691687-Acute-Phase Proteins,
pubmed-meshheading:7691687-Base Sequence,
pubmed-meshheading:7691687-Carcinoma, Hepatocellular,
pubmed-meshheading:7691687-Consensus Sequence,
pubmed-meshheading:7691687-DNA,
pubmed-meshheading:7691687-Humans,
pubmed-meshheading:7691687-Interleukin-6,
pubmed-meshheading:7691687-Liver,
pubmed-meshheading:7691687-Molecular Sequence Data,
pubmed-meshheading:7691687-Pancreas,
pubmed-meshheading:7691687-RNA, Messenger,
pubmed-meshheading:7691687-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:7691687-Transcription, Genetic,
pubmed-meshheading:7691687-Trypsin Inhibitor, Kazal Pancreatic,
pubmed-meshheading:7691687-Tumor Cells, Cultured
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pubmed:year |
1993
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pubmed:articleTitle |
Identification of the IL-6-responsive element in an acute-phase-responsive human pancreatic secretory trypsin inhibitor-encoding gene.
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pubmed:affiliation |
Department of Surgery II, Osaka University Medical School, Japan.
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pubmed:publicationType |
Journal Article
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