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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-10-21
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pubmed:databankReference |
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pubmed:abstractText |
Three cDNA clones homologous to rat neuronal nicotinic receptors were identified in a human brain stem library by screening at low stringency with a mixture of rat alpha 5, beta 2 and beta 4 subunit probes. Positive clones were further analysed by hybridisation at higher stringency with individual rat nicotinic receptor probes. One positive clone was found to encode a protein that exhibited characteristic features of a member of the ligand-gated ion channel class of protein subunits, and showed 89% homology with a rat beta 3 neuronal nicotinic receptor subunit. Northern blots demonstrated the presence of a 1.7 kb transcript in RNA extracted from adult human Pons. This clone has therefore been designated the human beta 3 neuronal nicotinic receptor subunit.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0304-3940
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
155
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
136-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7690916-Amino Acid Sequence,
pubmed-meshheading:7690916-Animals,
pubmed-meshheading:7690916-Brain Stem,
pubmed-meshheading:7690916-Cloning, Molecular,
pubmed-meshheading:7690916-DNA,
pubmed-meshheading:7690916-Gene Library,
pubmed-meshheading:7690916-Humans,
pubmed-meshheading:7690916-Ion Channel Gating,
pubmed-meshheading:7690916-Ion Channels,
pubmed-meshheading:7690916-Molecular Sequence Data,
pubmed-meshheading:7690916-Nerve Tissue Proteins,
pubmed-meshheading:7690916-Neurons,
pubmed-meshheading:7690916-Pons,
pubmed-meshheading:7690916-RNA, Messenger,
pubmed-meshheading:7690916-Rats,
pubmed-meshheading:7690916-Receptors, Nicotinic,
pubmed-meshheading:7690916-Sequence Alignment,
pubmed-meshheading:7690916-Sequence Homology, Amino Acid,
pubmed-meshheading:7690916-Sequence Homology, Nucleic Acid,
pubmed-meshheading:7690916-Species Specificity
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pubmed:year |
1993
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pubmed:articleTitle |
Molecular cloning of a human neuronal nicotinic acetylcholine receptor beta 3-like subunit.
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pubmed:affiliation |
Sandoz Institute for Medical Research, London, UK.
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pubmed:publicationType |
Journal Article,
Comparative Study
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