pubmed-article:7690869 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:7690869 | lifeskim:mentions | umls-concept:C0681814 | lld:lifeskim |
pubmed-article:7690869 | lifeskim:mentions | umls-concept:C0872303 | lld:lifeskim |
pubmed-article:7690869 | lifeskim:mentions | umls-concept:C2728259 | lld:lifeskim |
pubmed-article:7690869 | pubmed:issue | 1-2 | lld:pubmed |
pubmed-article:7690869 | pubmed:dateCreated | 1993-10-21 | lld:pubmed |
pubmed-article:7690869 | pubmed:abstractText | A Macintosh-based system is described for performing instrument control, data acquisition and storage operations in single-electrode whole-cell voltage clamp experiments. The system consists of a commercially available voltage clamp amplifier, multifunction input/output (I/O) board, graphical programming language (LabVIEW 2) and custom built 'virtual instrument' (VI). The I/O board is capable of fast (up to 110 ksample/s) multichannel analog-to-digital (A/D) conversion with 12-bit resolution. It can control the gain settings of the clamp unit through digital I/O lines and generate the P/N leak subtraction protocol to eliminate the linear portion of capacitive currents using analog output voltages and gating pulses. Complete voltage clamp protocols can be implemented using the on-screen front panel controls of the VI. It enables the user to visualize the acquired data, to graph sets of current-voltage (I-V) relations or to fit single-exponential functions to one current trace. To evaluate the adequacy of whole-cell recording, the total membrane capacitance (Cm), the series resistance (Rs) and the time constant (tau c) of the decay of the capacitive current are calculated using the single-exponential function fit to the data. The system is particularly well suited to the study of large quantities of transmembrane I-V relationships. Source code for the crucial elements of the VI as well as sample recordings from a cultured spinal cord neuron, illustrating system operation, are presented. | lld:pubmed |
pubmed-article:7690869 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:7690869 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:7690869 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:7690869 | pubmed:language | eng | lld:pubmed |
pubmed-article:7690869 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:7690869 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:7690869 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:7690869 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:7690869 | pubmed:month | Jun | lld:pubmed |
pubmed-article:7690869 | pubmed:issn | 0165-0270 | lld:pubmed |
pubmed-article:7690869 | pubmed:author | pubmed-author:WilcoxG LGL | lld:pubmed |
pubmed-article:7690869 | pubmed:author | pubmed-author:BudaiDD | lld:pubmed |
pubmed-article:7690869 | pubmed:author | pubmed-author:KehlL JLJ | lld:pubmed |
pubmed-article:7690869 | pubmed:author | pubmed-author:PoliacG IGI | lld:pubmed |
pubmed-article:7690869 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:7690869 | pubmed:volume | 48 | lld:pubmed |
pubmed-article:7690869 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:7690869 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:7690869 | pubmed:pagination | 65-74 | lld:pubmed |
pubmed-article:7690869 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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pubmed-article:7690869 | pubmed:year | 1993 | lld:pubmed |
pubmed-article:7690869 | pubmed:articleTitle | An iconographic program for computer-controlled whole-cell voltage clamp experiments. | lld:pubmed |
pubmed-article:7690869 | pubmed:affiliation | Department of Pharmacology, University of Minnesota, Medical School, Minneapolis 55455. | lld:pubmed |
pubmed-article:7690869 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:7690869 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
pubmed-article:7690869 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |