Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-10-12
pubmed:abstractText
We developed a non-radioisotopic quantitative RT-PCR method with high sensitivity and reproducibility. The results of this RT-PCR were in agreement with those of the Northern blot analysis. We measured the mRNA levels of beta-actin, transferrin receptor, and two cell cycle-related genes, cyclin B and cdc25, in early mouse embryos by the RT-PCR. In late two-cell stage embryos, beta-actin, transferrin receptor and cyclin B mRNA levels were 10-20% of those in MII stage oocytes. In contrast, the cdc25 mRNA levels were not different between these stages. When we cultured mouse embryos, the presence of an RNA polymerase inhibitor, alpha-amanitin, in the medium did not affect the mRNA levels at the two-cell stage, indicating that most of the detected mRNAs in two-cell embryos were maternally derived. These results suggest that the rate of mRNA degradation is different between cyclin B and cdc25 during early embryogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
195
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
769-75
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:7690561-Actins, pubmed-meshheading:7690561-Animals, pubmed-meshheading:7690561-Base Sequence, pubmed-meshheading:7690561-Blotting, Northern, pubmed-meshheading:7690561-Bone Marrow, pubmed-meshheading:7690561-Cell Line, pubmed-meshheading:7690561-Cyclins, pubmed-meshheading:7690561-Embryo, Mammalian, pubmed-meshheading:7690561-Embryonic and Fetal Development, pubmed-meshheading:7690561-Female, pubmed-meshheading:7690561-Macrophage Colony-Stimulating Factor, pubmed-meshheading:7690561-Mice, pubmed-meshheading:7690561-Mice, Inbred Strains, pubmed-meshheading:7690561-Molecular Sequence Data, pubmed-meshheading:7690561-Oligodeoxyribonucleotides, pubmed-meshheading:7690561-Oligonucleotides, Antisense, pubmed-meshheading:7690561-Polymerase Chain Reaction, pubmed-meshheading:7690561-Protein Biosynthesis, pubmed-meshheading:7690561-RNA, Messenger, pubmed-meshheading:7690561-RNA-Directed DNA Polymerase, pubmed-meshheading:7690561-Receptors, Transferrin, pubmed-meshheading:7690561-cdc25 Phosphatases
pubmed:year
1993
pubmed:articleTitle
Non-radioisotopic quantitative RT-PCR to detect changes in mRNA levels during early mouse embryo development.
pubmed:affiliation
Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, Japan.
pubmed:publicationType
Journal Article