7689152

pubmed:Citation

9

Genes which mediate odorant signal transduction are expressed at high levels in neurons of the olfactory epithelium. The molecular mechanism governing the restricted expression of these genes likely involves tissue-specific DNA binding proteins which coordinately activate transcription through sequence-specific interactions with olfactory promoter regions. We have identified binding sites for the olfactory neuron-specific transcription factor, Olf-1, in the sequences surrounding the transcriptional initiation site of five olfactory neuron-specific genes. The Olf-1 binding sites described define the consensus sequence YTCCCYRGGGAR. In addition, we have identified a second binding site, the U site, in the olfactory cyclic nucleotide gated channel and type III cyclase promoters, which binds factors present in all tissue examined. These experiments support a model in which expression of Olf-1 in the sensory neurons coordinately activates a set of olfactory neuron-specific genes. Furthermore, expression of a subset of these genes may be modulated by additional binding factors.

eng

IM

MEDLINE

0270-7306

Print

NLM

5805-13

pubmed-meshheading:7689152-Adenylate Cyclase, pubmed-meshheading:7689152-Animals, pubmed-meshheading:7689152-Base Sequence, pubmed-meshheading:7689152-Cloning, Molecular, pubmed-meshheading:7689152-DNA-Binding Proteins, pubmed-meshheading:7689152-Gene Expression Regulation, pubmed-meshheading:7689152-Genes, pubmed-meshheading:7689152-Ion Channels, pubmed-meshheading:7689152-Molecular Sequence Data, pubmed-meshheading:7689152-Nerve Tissue Proteins, pubmed-meshheading:7689152-Olfactory Marker Protein, pubmed-meshheading:7689152-Olfactory Pathways, pubmed-meshheading:7689152-Oligodeoxyribonucleotides, pubmed-meshheading:7689152-Promoter Regions, Genetic, pubmed-meshheading:7689152-Rats, pubmed-meshheading:7689152-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:7689152-Restriction Mapping, pubmed-meshheading:7689152-Sensory Receptor Cells, pubmed-meshheading:7689152-Sequence Alignment, pubmed-meshheading:7689152-Signal Transduction, pubmed-meshheading:7689152-Smell, pubmed-meshheading:7689152-Trans-Activators, pubmed-meshheading:7689152-Transcription Factors

Genes encoding components of the olfactory signal transduction cascade contain a DNA binding site that may direct neuronal expression.

Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.