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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1993-9-1
|
| pubmed:abstractText |
We have investigated the ability of compound 48/80 and of histamine H1 and H2 receptor antagonists to inhibit toluene diisocyanate-induced contractions in isolated guinea-pig bronchi. Compound 48/80 (100 micrograms/ml) significantly inhibited toluene diisocyanate-induced contractions. By contrast, the two histamine H1 and H2 receptor antagonists, chlorpheniramine (10 microM) and cimetidine, (10 microM) did not affect toluene diisocyanate-induced contractions, but significantly inhibited contractions induced by exogenously applied histamine (100 microM) and by 48/80. We investigated which mechanisms 48/80 used to inhibit toluene diisocyanate-induced contractions, paying particular attention to the possible involvement of capsaicin-sensitive primary afferents. In vitro capsaicin desensitization (10 microM for 30 min followed by washing) significantly reduced compound 48/80-induced contractions. A capsaicin-resistant component of contraction was also evident. Ruthenium red (3 microM), an inorganic dye which acts as a selective functional antagonist of capsaicin, did not affect 48/80-induced contraction. MEN 10,207 (Tyr5,D-Trp6,8,9,Arg10)-neurokinin A (4-10) (3 microM) a selective antagonist of NK2-tachykinin receptors significantly reduced 48/80-induced contractions. These results show that compound 48/80 inhibits toluene diisocyanate-induced contractions in isolated guinea-pig bronchi. It is likely that two mechanisms are involved in the inhibition: (1) the release of mediators other than histamine by mast cells, (2) an effect of 48/80 on sensory nerves.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Capsaicin,
http://linkedlifedata.com/resource/pubmed/chemical/Glycopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine H1 Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine H2 Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Neprilysin,
http://linkedlifedata.com/resource/pubmed/chemical/Neurokinin A,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Ruthenium Red,
http://linkedlifedata.com/resource/pubmed/chemical/Toluene 2,4-Diisocyanate,
http://linkedlifedata.com/resource/pubmed/chemical/neurokinin A (4-10)...,
http://linkedlifedata.com/resource/pubmed/chemical/p-Methoxy-N-methylphenethylamine,
http://linkedlifedata.com/resource/pubmed/chemical/phosphoramidon
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0014-2999
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
248
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
67-73
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7687959-Animals,
pubmed-meshheading:7687959-Bronchoconstriction,
pubmed-meshheading:7687959-Capsaicin,
pubmed-meshheading:7687959-Cell Degranulation,
pubmed-meshheading:7687959-Glycopeptides,
pubmed-meshheading:7687959-Guinea Pigs,
pubmed-meshheading:7687959-Histamine H1 Antagonists,
pubmed-meshheading:7687959-Histamine H2 Antagonists,
pubmed-meshheading:7687959-Male,
pubmed-meshheading:7687959-Mast Cells,
pubmed-meshheading:7687959-Muscle, Smooth,
pubmed-meshheading:7687959-Muscle Contraction,
pubmed-meshheading:7687959-Neprilysin,
pubmed-meshheading:7687959-Neurokinin A,
pubmed-meshheading:7687959-Peptide Fragments,
pubmed-meshheading:7687959-Ruthenium Red,
pubmed-meshheading:7687959-Toluene 2,4-Diisocyanate,
pubmed-meshheading:7687959-p-Methoxy-N-methylphenethylamine
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
The effect of compound 48/80 on contractions induced by toluene diisocyanate in isolated guinea-pig bronchus.
|
| pubmed:affiliation |
Institute of Occupational Medicine, University of Padua, Italy.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|