Linked Life Data

7687441

Source:http://linkedlifedata.com/resource/pubmed/id/7687441

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-8-17
pubmed:abstractText
We examined whether ouabain activities phospholipases and reinforces contraction force of papillary muscles through resultant second messengers. 2-Nitro-4-carboxyphenyl-N,N-diphenylcarbamate (NCDC), the inhibitor of phospholipase C (PLC), abolished the ouabain inotropy in rabbit papillary muscles. Calphostin C, the specific inhibitor of protein kinase C (PKC), also depressed the ouabain inotropy. 12-O-Tetradecanoylphorbor-13-acetate (TPA), the specific activator of PKC, enhanced the beat-to-beat phasic contractility at low concentrations. Radioenzymatic assay revealed that ouabain treatment doubled diacylglycerol (DG) content in excised papillary muscles. We concluded that ouabain activates PLC, and the resultant second messenger, DG, augments the cardiac contraction force through activation of PKC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
194
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
72-8
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Involvement of phosphoinositide turnover in ouabain inotropism.
pubmed:affiliation
Department of Physiology, Iwate Medical University School of Medicine, Japan.
pubmed:publicationType
Journal Article, In Vitro