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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1993-8-12
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pubmed:abstractText |
The encephalitogenic potential of a segment of myelin basic protein in experimental autoimmune encephalomyelitis is not always mirrored by the ability of the peptide to mediate in vitro activation of encephalitogenic T cells. Recent studies from our laboratory have demonstrated that the responsiveness of Ag-specific T cells in experimental autoimmune encephalomyelitis is determined not exclusively by Ag but also by the nature of the APC. By varying APC during the in vitro selection of T cells, we could generate distinct sets of rat encephalitogenic T cells, as evidenced by the diversity of TCR usage. Here we establish the importance of APC in the activation of rat encephalitogenic T cells by myelin basic protein peptides. Peptides 69-84-Gly and (P80)68-86, which lacked stimulatory activity toward many encephalitogenic T cells in our proliferation assay when standard APC were used, become strongly stimulatory in the presence of less commonly used APC, i.e., an Ia+ T cell clone (LOA) or an Ia-inducible rat glial cell clone (F10). Nonstimulatory APC failed to activate encephalitogenic T cells even when major cytokines were added, suggesting that these cytokines are not among the factors limiting the activating potential of the APC. Thus, whether or not an immunocompetent T cell can be activated by a given Ag in an autoimmune response may be determined by the properties of APC. This finding has implications for current research efforts to identify pathogenic self proteins.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
151
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
111-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7686928-Amino Acid Sequence,
pubmed-meshheading:7686928-Animals,
pubmed-meshheading:7686928-Antigen-Presenting Cells,
pubmed-meshheading:7686928-Clone Cells,
pubmed-meshheading:7686928-Cytokines,
pubmed-meshheading:7686928-Encephalomyelitis, Autoimmune, Experimental,
pubmed-meshheading:7686928-Histocompatibility Antigens Class II,
pubmed-meshheading:7686928-Immunization, Passive,
pubmed-meshheading:7686928-Lymphocyte Activation,
pubmed-meshheading:7686928-Molecular Sequence Data,
pubmed-meshheading:7686928-Myelin Basic Proteins,
pubmed-meshheading:7686928-Peptides,
pubmed-meshheading:7686928-Rats,
pubmed-meshheading:7686928-Rats, Inbred Lew,
pubmed-meshheading:7686928-Spleen,
pubmed-meshheading:7686928-T-Lymphocytes
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pubmed:year |
1993
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pubmed:articleTitle |
Major role of antigen-presenting cells in the response of rat encephalitogenic T cells to myelin basic proteins.
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pubmed:affiliation |
Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38101.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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