Linked Life Data

7686855

Source:http://linkedlifedata.com/resource/pubmed/id/7686855

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1993-8-9
pubmed:abstractText
We have investigated the immune response against the Plasmodium falciparum gametocyte-specific antigen Pf11-1. This megadalton parasite molecule has been implicated in the process of erythrocyte rupture during gametogenesis. The molecule is composed in great part of degenerated nonapeptide motifs which are tandemly repeated several hundred times. A computer algorithm searching for T sites predicted that the entire repeat region of the Pf11-1 represents potential T cell antigenic major histocompatibility complex class II-binding sites. To test this hypothesis, synthetic peptides corresponding to two nonamer subtype repeats, differing only at two amino acid positions, were used to immunize congenic mouse strains. Both peptides were shown to contain both B and T cell epitopes. The immune response is restricted to the H-2d and H-2k haplotypes. The T cell response against the peptides appeared to be highly specific, clearly discriminating between the two similar nonamer repeat sequences, whereas the humoral response produced cross-reacting antibodies. We also investigated the humoral and T cell reactivities of P. falciparum-primed individuals in West Africa against the synthetic Pf11-1 peptides. Among 51 individuals 35 had antibodies to at least one of the two peptides and a majority of them (28) had antibodies reacting with both peptides. The cellular response was analyzed by [3H]thymidine incorporation or interferon-gamma release. There was considerable variation in the response to the two peptides. Among the human samples 36% responded to one repeat subtype, while only 13% responded to the second subtype. Interestingly, in individual donors the T cell response to both peptides are associated, suggesting that, as shown for mice, the response is restricted by a genetic element. The data obtained on the two subtypes of the nonamer repeat region suggest that the entire Pf11-1 molecule might induce an unusually heterogenous B and T cell response during natural infection in man.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1574-81
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7686855-Adolescent, pubmed-meshheading:7686855-Adult, pubmed-meshheading:7686855-Amino Acid Sequence, pubmed-meshheading:7686855-Animals, pubmed-meshheading:7686855-Antibodies, Protozoan, pubmed-meshheading:7686855-Antigens, Protozoan, pubmed-meshheading:7686855-B-Lymphocytes, pubmed-meshheading:7686855-Child, pubmed-meshheading:7686855-Child, Preschool, pubmed-meshheading:7686855-Epitopes, pubmed-meshheading:7686855-Female, pubmed-meshheading:7686855-Humans, pubmed-meshheading:7686855-Malaria, pubmed-meshheading:7686855-Male, pubmed-meshheading:7686855-Mice, pubmed-meshheading:7686855-Peptides, pubmed-meshheading:7686855-Plasmodium falciparum, pubmed-meshheading:7686855-Protozoan Proteins, pubmed-meshheading:7686855-Repetitive Sequences, Nucleic Acid, pubmed-meshheading:7686855-T-Lymphocytes
pubmed:year
1993
pubmed:articleTitle
Immune response in mouse and malaria-exposed humans to peptides derived from Pf11-1, a highly repetitive megadalton protein of Plasmodium falciparum.
pubmed:affiliation
Unité de Parasitologie Expérimentale, CNRS URA 361, Institut Pasteur, Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't