Linked Life Data

7686774

Source:http://linkedlifedata.com/resource/pubmed/id/7686774

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-8-9
pubmed:abstractText
A method for coupling bleomycin A5 to oligonucleotides is proposed. The reaction was carried out between an amino group of a spermidine residue of the Cu(II) complex of bleomycin A5 (Cu(II)Blm-RH) and a 5'-phosphate group of the oligonucleotides d(pCCAAACA) (I), d(pCGTCCTC) (II), d(pT)16 (III), d(pCAAACA) (IV), and d(pGCCAAACA) (V) activated with a mixture of triphenylphosphine and 2,2'-dipyridyl disulfide in the presence of 4-(N,N-dimethylamino)pyridine 1-oxide. The yields of the products Cu(II)Blm-R-d(pCCAAACA) (Ia), Cu(II)Blm-R-d(pCGTCCTC) (IIa), Cu(II)Blm-R-d(pT)16 (IIIa), Cu(II)Blm-R-d(pCAAACA) (IVa), and Cu(II)Blm-R-d(pGCCAAACA) (Va) were 60-80%. After removal of the Cu(II) ion from the bleomycin A5 oligonucleotide derivatives Ia-IIIa, compounds Ib-IIIb were obtained. Compounds Ia, IVa, Va, and Ib-IIIb were further used for modification of the target d(pTGTTTGGCGAAGGA) in the presence of Fe(II) ions and 2-mercaptoethanol. Site-specific cleavage of the target by Blm coupled to complementary oligonucleotides was demonstrated. It was shown that efficiency and position of cleavage of the complementary reagents Ia, Ib, IVa, and Va are determined by their oligonucleotide part while the action of thenoncomplementary reagents IIb and IIIb was similar to that of the free antibiotic.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1043-1802
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
189-93
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:articleTitle
Synthesis of bleomycin A5 oligonucleotide derivatives and site-specific cleavage of the DNA target.
pubmed:affiliation
Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences, Russia.
pubmed:publicationType
Journal Article