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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1993-7-22
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pubmed:abstractText |
Previously we found that interleukin 2 (IL-2) induces tyrosine phosphorylation and activation of the serine/threonine-specific kinase encoded by the raf-1 protooncogene in a T-cell line, CTLL-2. Here we extended these findings by exploring the effects of selective removal of phosphate from tyrosines in p72-74-Raf-1 kinase that had been immunoprecipitated from IL-2-stimulated CTLL-2 cells. Treatment in vitro of IL-2-activated Raf-1 with the tyrosine-specific phosphatases CD45 and TCPTP (formerly called T-cell protein tyrosine phosphatase) reduced Raf kinase activity to nearly baseline levels. This effect was completely inhibited by the phosphatase inhibitor sodium orthovanadate. In contrast, treatment of Raf-1 with a serine/threonine-specific phosphatase, protein phosphatase 1 (PP-1), resulted in a more modest decrease in Raf in vitro kinase activity, and this effect was prevented by okadaic acid. Two-dimensional phosphoamino acid analysis confirmed the selective removal of phosphate from tyrosine by CD45 and from serine and threonine by PP-1. The immunoreactivity of p72-74-Raf-1 with anti-phosphotyrosine antibodies was also completely abolished by treatment with CD45 in the absence but not in the presence of sodium orthovanadate. These findings provide evidence that the IL-2-stimulated phosphorylation of Raf-1 on tyrosines plays an important role in upregulating the activity of this serine/threonine-specific kinase in CTLL-2 cells and, as such, provides a model system for studying the transfer of growth factor-initiated signals from protein tyrosine kinases to serine/threonine-specific kinases.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-145543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-1524574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-1631559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-1639773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-1655887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-1693184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-1696599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-1698619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-1700980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-1708096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-1845931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-1888699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-1990291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-1996324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-2154696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-2158859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-2170413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-2197271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-2303462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-2475255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-2785715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-2787319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-2834386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-2853967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-2994296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-3011033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-3057494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-3259228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-3903508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-6367046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685905-6975300
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ethers, Cyclic,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Okadaic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoprotein Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Phosphatase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-raf,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Vanadates
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5544-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7685905-Animals,
pubmed-meshheading:7685905-Cell Line,
pubmed-meshheading:7685905-Ethers, Cyclic,
pubmed-meshheading:7685905-Interleukin-2,
pubmed-meshheading:7685905-Kinetics,
pubmed-meshheading:7685905-Mice,
pubmed-meshheading:7685905-Okadaic Acid,
pubmed-meshheading:7685905-Phosphates,
pubmed-meshheading:7685905-Phosphoprotein Phosphatases,
pubmed-meshheading:7685905-Phosphorylation,
pubmed-meshheading:7685905-Phosphotyrosine,
pubmed-meshheading:7685905-Protein Phosphatase 1,
pubmed-meshheading:7685905-Protein-Serine-Threonine Kinases,
pubmed-meshheading:7685905-Proto-Oncogene Proteins,
pubmed-meshheading:7685905-Proto-Oncogene Proteins c-raf,
pubmed-meshheading:7685905-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:7685905-Tyrosine,
pubmed-meshheading:7685905-Vanadates
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pubmed:year |
1993
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pubmed:articleTitle |
Interleukin 2 regulates Raf-1 kinase activity through a tyrosine phosphorylation-dependent mechanism in a T-cell line.
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pubmed:affiliation |
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104-6082.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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