Linked Life Data

7685351

Source:http://linkedlifedata.com/resource/pubmed/id/7685351

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
1993-7-13
pubmed:abstractText
By means of selective extraction in a Ca(2+)-chelating medium and immunoblotting, four annexins (I, II, V, and VI) were identified in both isolated rat renal glomeruli and rat glomerular mesangial cells. Upon 32P labeling of these cells in culture, annexin I was immunoprecipitated using a specific polyclonal antibody and was found to incorporate radioactivity in a constitutive manner. However, as with epidermal growth factor (200 ng/ml), addition of angiotensin II (10(-7) M), arginine-vasopressin (10(-7) M), or endothelin I (10(-7) M) resulted in a 2-3-fold stimulation of annexin I phosphorylation. The basal phosphorylation as well as the stimulating effect of angiotensin II were also detected by immunoblotting annexin extracts using an antiphosphotyrosine antibody. In addition, among various phosphotyrosyl proteins isolated from EGTA extracts by adsorption onto an anti-phosphotyrosine antibody, annexin I was specifically recognized by Western blotting using a monoclonal anti-annexin I antibody, and displayed the same increase upon cell stimulation with angiotensin II. Moreover, thin layer chromatographic analysis of phosphoamino acids present in immunoprecipitated [32P]annexin I showed an exclusive labeling of phosphotyrosine residue(s). Finally, the effect of angiotensin II was detectable after 10 min, maximal at 6 h, and present until 12 h of incubation. Using 12-h stimulation, tyrosine phosphorylation of annexin I displayed a maximum at 10(-7) to 10(-6) M angiotensin II. These data report for the first time the stimulation of annexin I tyrosine phosphorylation by biologically active peptides acting via receptors belonging to the superfamily of seven hydrophobic domain, G-protein-linked receptors, which lack an intrinsic protein tyrosine kinase. This suggests a possible role of annexin I in the mitogenic effect of angiotensin II, arginine-vasopressin, and endothelin I, which was previously observed on rat glomerular mesangial cells as well as on other cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
268
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12805-11
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7685351-Angiotensin II, pubmed-meshheading:7685351-Animals, pubmed-meshheading:7685351-Annexin A1, pubmed-meshheading:7685351-Calcium, pubmed-meshheading:7685351-Cells, Cultured, pubmed-meshheading:7685351-Chromatography, Affinity, pubmed-meshheading:7685351-Egtazic Acid, pubmed-meshheading:7685351-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:7685351-Glomerular Mesangium, pubmed-meshheading:7685351-Male, pubmed-meshheading:7685351-Molecular Weight, pubmed-meshheading:7685351-Phosphates, pubmed-meshheading:7685351-Phospholipids, pubmed-meshheading:7685351-Phosphoproteins, pubmed-meshheading:7685351-Phosphorus Radioisotopes, pubmed-meshheading:7685351-Phosphorylation, pubmed-meshheading:7685351-Phosphotyrosine, pubmed-meshheading:7685351-Rats, pubmed-meshheading:7685351-Rats, Wistar, pubmed-meshheading:7685351-Tyrosine
pubmed:year
1993
pubmed:articleTitle
Sustained effect of angiotensin II on tyrosine phosphorylation of annexin I in glomerular mesangial cells.
pubmed:affiliation
Institut National de la Santé et de la Recherche Médicale, Unité 326, Hôpital Purpan, Toulouse, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't