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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1993-7-2
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pubmed:abstractText |
T cells have been shown to express CD26, a known ectoenzyme with dipeptidyl peptidase IV (DPPIV; EC 3.4.14.5) activity in its extracellular domain. CD26 can also deliver a second costimulatory signal and contribute to T-cell activation. In an earlier study, we established CD26-transfected Jurkat T-cell lines and demonstrated that monoclonal antibody-mediated crosslinking of CD26 and CD3 induced interleukin 2 (IL-2) production. To determine the contribution of DPPIV enzymatic activity to the costimulatory activity of CD26, human CD26 cDNA was mutated so that active-site serine was replaced by alanine. The mutant CD26 antigen lacked DPPIV enzyme activity but still retained reactivity with three anti-CD26 monoclonal antibodies directed against distinct epitopes of CD26. After stimulation with a combination of anti-CD26 and anti-CD3 antibodies, wild-type CD26 (DPPIV+)-transfected Jurkat cells produced substantially more IL-2 than did mutant CD26 (DPPIV-) or CD26- control transfectants. Nevertheless, the mutant CD26-transfected cells still produced significantly more IL-2 than did CD26- control transfectants. These results suggest that DPPIV activity plays an important but not absolute role in the co-stimulatory activity of CD26 in this system. We also found that wild-type CD26 (DPPIV+) transfectants produced more IL-2 than mutant CD26 (DPPIV-)-transfected cells or CD26- control transfectants when triggered by stimuli not involving CD26, such as anti-CD3 and phorbol ester. These results suggest that the DPPIV activity of CD26 functions to augment the cellular responses of CD26-transfected Jurkat cells to external stimuli mediated by CD26 and/or the CD3/T-cell receptor complex, thus enhancing IL-2 production.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-1347701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-1352530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-1358482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-1371526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-1371820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-1671716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-1680916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-1969875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-1970666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-1971293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-1979581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-2147918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-2152856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-2466591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-2474605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-2479677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-2564215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-2564857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-2824653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-2891539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-3100637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-3136396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-3323813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-3507689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-3881765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-6205075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-962103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7685106-962853
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4586-90
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:7685106-Antigens, CD3,
pubmed-meshheading:7685106-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:7685106-Base Sequence,
pubmed-meshheading:7685106-Dipeptidyl Peptidase 4,
pubmed-meshheading:7685106-Dipeptidyl-Peptidases and Tripeptidyl-Peptidases,
pubmed-meshheading:7685106-Epitopes,
pubmed-meshheading:7685106-Humans,
pubmed-meshheading:7685106-Interleukin-2,
pubmed-meshheading:7685106-Lymphocyte Activation,
pubmed-meshheading:7685106-Molecular Sequence Data,
pubmed-meshheading:7685106-Mutagenesis, Site-Directed,
pubmed-meshheading:7685106-Oligodeoxyribonucleotides,
pubmed-meshheading:7685106-Structure-Activity Relationship,
pubmed-meshheading:7685106-T-Lymphocytes,
pubmed-meshheading:7685106-Tetradecanoylphorbol Acetate,
pubmed-meshheading:7685106-Transfection,
pubmed-meshheading:7685106-Tumor Cells, Cultured
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pubmed:year |
1993
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pubmed:articleTitle |
The costimulatory activity of the CD26 antigen requires dipeptidyl peptidase IV enzymatic activity.
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pubmed:affiliation |
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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