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rdf:type |
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lifeskim:mentions |
umls-concept:C0015272,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0036098,
umls-concept:C0205231,
umls-concept:C0205314,
umls-concept:C0332307,
umls-concept:C0597427,
umls-concept:C0679622,
umls-concept:C0998476,
umls-concept:C1171362,
umls-concept:C1444754,
umls-concept:C1515670,
umls-concept:C1882417
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pubmed:issue |
16
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pubmed:dateCreated |
1993-7-7
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pubmed:databankReference |
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pubmed:abstractText |
The secretion from the salivary glands in the dipteran Chironomus tentans has previously been shown to contain secretory proteins encoded by a set of related genes belonging to the Balbiani ring multigene family. Here we describe the characterization of a gene for an additional secretory protein of a novel type. This sp240/420 gene is built from virtually identical 477-base pair repeats organized in tandem. The gene exhibits extensive length polymorphism since allelic length variants with from 12 to 22 repeats were recorded. Antibodies have been raised against the gene product, and it is shown also that the protein varies in size between 240 and 420 kDa. The protein is rich in serine and threonine residues (30%) and has N-linked carbohydrate chains, presumably on each repeat. Despite careful preparation of the gland lumen proteins, the sp240/420 protein was found to form protein ladders of regularly degraded protein, suggesting that this degradation occurs naturally in the salivary glands. Although all previously characterized salivary gland secretory protein genes in C. tentans belong to the same multigene family, the sp240/420 gene appears to represent a novel, unrelated type of repetitive gene. The chromosomal location of the gene was mapped and was found to reside in region 17 on chromosome I.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
268
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pubmed:geneSymbol |
sp240/420
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11888-93
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7685024-Amino Acid Sequence,
pubmed-meshheading:7685024-Animals,
pubmed-meshheading:7685024-Base Composition,
pubmed-meshheading:7685024-Base Sequence,
pubmed-meshheading:7685024-Blotting, Northern,
pubmed-meshheading:7685024-Blotting, Southern,
pubmed-meshheading:7685024-Blotting, Western,
pubmed-meshheading:7685024-Chironomidae,
pubmed-meshheading:7685024-Chromosome Mapping,
pubmed-meshheading:7685024-DNA,
pubmed-meshheading:7685024-In Situ Hybridization,
pubmed-meshheading:7685024-Insect Proteins,
pubmed-meshheading:7685024-Molecular Sequence Data,
pubmed-meshheading:7685024-Multigene Family,
pubmed-meshheading:7685024-Polymerase Chain Reaction,
pubmed-meshheading:7685024-Polymorphism, Genetic,
pubmed-meshheading:7685024-RNA,
pubmed-meshheading:7685024-Recombinant Fusion Proteins,
pubmed-meshheading:7685024-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:7685024-Salivary Glands,
pubmed-meshheading:7685024-Salivary Proteins and Peptides
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pubmed:year |
1993
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pubmed:articleTitle |
A repetitive secretory protein gene of a novel type in Chironomus tentans is specifically expressed in the salivary glands and exhibits extensive length polymorphism.
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pubmed:affiliation |
Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, Stockholm, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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