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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
1993-7-7
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pubmed:abstractText |
Insulin-like growth factor (IGF) I has important growth regulatory functions in normal growth and development. IGF-I is also a mitogen for a number of cancer cell lines; however, its autocrine effect has not been well established. In this study, the expression of IGF-I, its receptor, and its major serum-binding protein were examined in 5 normal human mesothelial (NHM) cell samples and 11 pleural mesothelioma cell lines. All NHM cells and mesothelioma cell lines expressed IGF-I, IGF-binding protein 3 (IGFBP-3), and IGF-I receptor mRNA by either Northern blot or reverse transcription polymerase chain reaction analysis. IGF-I (0.136 +/- 0.024 ng/ml, mean +/- SEM) and IGFBP-3 (18.5 +/- 3.2 ng/ml) proteins were readily detected in the conditioned medium of mesothelioma cell lines but were not greater than corresponding measurements in that of NHM cells (IGF-I, 0.120 +/- 0.080 ng/ml; IGFBP-3, 15.9 +/- 1.3 ng/ml). Exogenous recombinant IGF-I stimulated cell proliferation of NHM cells, demonstrating the presence of a functional IGF-I receptor. Our results suggest that IGF-I may function as an autocrine growth stimulus in normal proliferating mesothelial cells, which may contribute to their malignant transformation.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, IGF Type 1
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0008-5472
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
53
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2858-64
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7684950-Carrier Proteins,
pubmed-meshheading:7684950-Cell Division,
pubmed-meshheading:7684950-Epithelial Cells,
pubmed-meshheading:7684950-Epithelium,
pubmed-meshheading:7684950-Humans,
pubmed-meshheading:7684950-Insulin-Like Growth Factor Binding Proteins,
pubmed-meshheading:7684950-Insulin-Like Growth Factor I,
pubmed-meshheading:7684950-Mesothelioma,
pubmed-meshheading:7684950-Pleural Neoplasms,
pubmed-meshheading:7684950-RNA, Messenger,
pubmed-meshheading:7684950-Receptor, IGF Type 1,
pubmed-meshheading:7684950-Tumor Cells, Cultured
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pubmed:year |
1993
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pubmed:articleTitle |
Normal human mesothelial cells and mesothelioma cell lines express insulin-like growth factor I and associated molecules.
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pubmed:affiliation |
Chest Service, Bellevue Hospital Center, New York University Medical Center, New York 10016.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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