Linked Life Data

7684700

Source:http://linkedlifedata.com/resource/pubmed/id/7684700

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1993-6-29
pubmed:abstractText
Cells of the murine interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) factor-dependent line, FDC-P1, express the tyrosine kinase receptor, c-kit. The ligand for c-kit, steel factor (SLF), encoded by the steel (Sl) locus, is produced as both membrane-bound and soluble forms by fibroblastoid cells. Fibroblasts derived from normal (+/+) WCB6F1 mice are known to produce both forms of SLF and were able to support FDC-P1 cells in a contact-dependent manner in the presence of neutralizing anti-GM-CSF antiserum. In contrast, Sl/Sld mutant fibroblasts, which produce only a soluble form of SLF, were incapable of supporting FDC-P1 cells in the presence of GM-CSF antiserum. These results suggested that FDC-P1 cells were being supported on fibroblast layers by membrane-bound SLF. Attempts to grow FDC-P1 cells in high levels of soluble recombinant SLF to mimic the SLF-dependent response seen in co-culture experiments showed that cells which had been previously grown in GM-CSF or IL-3 were minimally responsive to SLF at concentrations up to 100 ng/mL. Although these cultures were not supported by SLF alone, the cells showed synergistic proliferative responses to SLF combined with suboptimal levels of GM-CSF or IL-3. FDC-P1 cells could, however, be adapted to grow in SLF alone by gradual substitution of SLF for GM-CSF over a period of 3 weeks. These cells showed 5.6- to 8.4-fold and 2.5-fold higher levels of c-kit mRNA than cells grown in GM-CSF or IL-3, respectively. Downregulation of surface c-kit protein was also seen in FDC-P1 cells grown in GM-CSF or IL-3 compared with cells grown in SLF. Although FDC-P1 cells propagated in SLF were more responsive to SLF, they were still able to proliferate as well in GM-CSF and IL-3 as the cells originally grown in the latter factors. Thus, functional downregulation of c-kit by GM-CSF and IL-3 was unidirectional.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0301-472X
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:geneSymbol
c-kit
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
761-8
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7684700-Animals, pubmed-meshheading:7684700-Bone Marrow, pubmed-meshheading:7684700-Bone Marrow Cells, pubmed-meshheading:7684700-Cell Communication, pubmed-meshheading:7684700-Cell Division, pubmed-meshheading:7684700-Cell Line, pubmed-meshheading:7684700-Dose-Response Relationship, Drug, pubmed-meshheading:7684700-Down-Regulation, pubmed-meshheading:7684700-Drug Synergism, pubmed-meshheading:7684700-Fibroblasts, pubmed-meshheading:7684700-Gene Expression Regulation, pubmed-meshheading:7684700-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:7684700-Hematopoietic Cell Growth Factors, pubmed-meshheading:7684700-Interleukin-3, pubmed-meshheading:7684700-Ligands, pubmed-meshheading:7684700-Mice, pubmed-meshheading:7684700-Proto-Oncogene Proteins, pubmed-meshheading:7684700-Proto-Oncogene Proteins c-kit, pubmed-meshheading:7684700-RNA, Messenger, pubmed-meshheading:7684700-Stem Cell Factor
pubmed:year
1993
pubmed:articleTitle
Responses of the murine myeloid cell line FDC-P1 to soluble and membrane-bound forms of steel factor (SLF).
pubmed:affiliation
Leukaemia Research Unit, Hanson Centre for Cancer Research, Adelaide, South Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't