Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
1993-6-24
pubmed:databankReference
pubmed:abstractText
Glyoxalase I (EC 4.4.1.5) catalyzes the transformation of methylglyoxal and glutathione to S-lactoylglutathione. We have isolated human cDNA clones encoding glyoxalase I from a phorbol myristate acetate-treated U937 cDNA library. This cDNA encodes a protein of 184 amino acids with a calculated M(r) of 20,719. The amino acid composition calculated from the deduced amino acid sequence agreed with that reported for glyoxalase I purified from human erythrocytes. The Escherichia coli cells carrying the expression vector of this cDNA acquired methylglyoxal resistance and the cell lysate showed the high activity of glyoxalase I. The amino acid sequence of human glyoxalase I exhibited 57% identity with Pseudomonas putida glyoxalase I at the C-terminal two-thirds, suggesting that the two enzymes may have originated from a common ancestor.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
268
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11217-21
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7684374-Amino Acid Sequence, pubmed-meshheading:7684374-Base Sequence, pubmed-meshheading:7684374-Blotting, Northern, pubmed-meshheading:7684374-Cloning, Molecular, pubmed-meshheading:7684374-DNA, Neoplasm, pubmed-meshheading:7684374-Escherichia coli, pubmed-meshheading:7684374-Gene Library, pubmed-meshheading:7684374-Humans, pubmed-meshheading:7684374-Lactoylglutathione Lyase, pubmed-meshheading:7684374-Molecular Sequence Data, pubmed-meshheading:7684374-Oligodeoxyribonucleotides, pubmed-meshheading:7684374-Poly A, pubmed-meshheading:7684374-Polymerase Chain Reaction, pubmed-meshheading:7684374-Pseudomonas putida, pubmed-meshheading:7684374-RNA, pubmed-meshheading:7684374-RNA, Messenger, pubmed-meshheading:7684374-Restriction Mapping, pubmed-meshheading:7684374-Sequence Homology, Amino Acid, pubmed-meshheading:7684374-Tetradecanoylphorbol Acetate, pubmed-meshheading:7684374-Tumor Cells, Cultured
pubmed:year
1993
pubmed:articleTitle
Human glyoxalase I. cDNA cloning, expression, and sequence similarity to glyoxalase I from Pseudomonas putida.
pubmed:affiliation
Kanagawa Academy of Science and Technology (KAST), Japan.
pubmed:publicationType
Journal Article, Comparative Study