Linked Life Data

7683181

Source:http://linkedlifedata.com/resource/pubmed/id/7683181

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-5-27
pubmed:databankReference
pubmed:abstractText
A fluorescently labeled version of a population-specific oligonucleotide hybridization probe was used to monitor the enrichment and isolation of a sulfate-reducing bacterium from a multispecies anaerobic bioreactor. The organism was originally identified as a molecular isolate that was phylogenetically related to Desulfovibrio vulgaris by amplification and sequencing of part of its 16S rRNA sequence. The sequence, in turn, was used to design a population-specific probe. The anaerobic medium used for the organism's enrichment and isolation was based on the physiological properties of the its closest relatives as identified by sequence comparisons. Of 30 isolates examined, only 3 hybridized with the probe. Nearly complete 16S rRNA sequences determined for each of these three isolates (i) had no mismatches with the probe target site, (ii) were identical to the amplified partial sequence of about 500 nucleotides and to one another in all other positions, and (iii) were 93.9% similar to that of D. vulgaris. In addition, one isolate chosen for further study (strain PT-2) had a substrate specificity comparable to that of D. vulgaris. These results confirmed that polymerase chain reaction amplification of 16S rRNA sequences from environmental samples can be accurate and can also provide phylogenetic information from which aspects of a population's physiology can be inferred.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-1376982, http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-14063318, http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-1688842, http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-1691827, http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-1709451, http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-1741613, http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-1967116, http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-2200342, http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-2252397, http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-2330053, http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-2413450, http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-2439888, http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-2466341, http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-2480344, http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-2646290, http://linkedlifedata.com/resource/pubmed/commentcorrection/7683181-6363901
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
682-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Monitoring the enrichment and isolation of sulfate-reducing bacteria by using oligonucleotide hybridization probes designed from environmentally derived 16S rRNA sequences.
pubmed:affiliation
Department of Veterinary Pathobiology, University of Illinois, Urbana 61801.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't