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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1993-5-27
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pubmed:abstractText |
The gIII glycoproteins of both bovine herpesvirus 1 (BHV 1) and pseudorabies virus (PrV) mediate the initial and dominant interactions between virus and permissive host cells. By studying virus binding to wild-type and heparin-deficient CHO cells, we demonstrated that the cellular heparin-like moieties play an essential role in BHV 1 and PrV gIII-mediated virus attachment. Subsequent studies were carried out to map the gIII structures that are responsible for heparin binding. First, based on the observation that BHV 1 and PrV are differentially sensitive to heparin inhibition of gIII-mediated attachment to cells, we conducted a gIII domain shuffling experiment. This involved the construction of a set of recombinant BHV 1 expressing BHV 1 and PrV gIII chimeras and then using the sensitivity to heparin inhibition as a means of mapping the potential heparin-binding regions on the gIII molecules. Next, we synthesized panels of partially overlapping BHV 1 and PrV gIII peptides and examined their reactivity to heparin. The results from these experiments demonstrated five heparin-binding sites between amino acid 129 and 310 of BHV 1 gIII and four heparin-binding sites between amino acid 90 and 275 of PrV gIII.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
194
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
233-43
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:7683156-Amino Acid Sequence,
pubmed-meshheading:7683156-Animals,
pubmed-meshheading:7683156-Binding, Competitive,
pubmed-meshheading:7683156-CHO Cells,
pubmed-meshheading:7683156-Cells, Cultured,
pubmed-meshheading:7683156-Cricetinae,
pubmed-meshheading:7683156-DNA Mutational Analysis,
pubmed-meshheading:7683156-Dogs,
pubmed-meshheading:7683156-Epitopes,
pubmed-meshheading:7683156-Genetic Vectors,
pubmed-meshheading:7683156-Heparin,
pubmed-meshheading:7683156-Herpesvirus 1, Bovine,
pubmed-meshheading:7683156-Herpesvirus 1, Suid,
pubmed-meshheading:7683156-Molecular Sequence Data,
pubmed-meshheading:7683156-Peptide Fragments,
pubmed-meshheading:7683156-Recombinant Proteins,
pubmed-meshheading:7683156-Transfection,
pubmed-meshheading:7683156-Viral Envelope Proteins,
pubmed-meshheading:7683156-Viral Plaque Assay,
pubmed-meshheading:7683156-Viral Proteins
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pubmed:year |
1993
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pubmed:articleTitle |
Mapping of heparin-binding structures on bovine herpesvirus 1 and pseudorabies virus gIII glycoproteins.
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pubmed:affiliation |
Veterinary Infectious Disease Organization, Saskatoon, Saskatchewan, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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