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rdf:type |
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lifeskim:mentions |
umls-concept:C0008633,
umls-concept:C0015295,
umls-concept:C0017337,
umls-concept:C0086418,
umls-concept:C0206473,
umls-concept:C0450429,
umls-concept:C0678594,
umls-concept:C0949765,
umls-concept:C1335671,
umls-concept:C1519249,
umls-concept:C1519595,
umls-concept:C1523987
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pubmed:issue |
16
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pubmed:dateCreated |
1993-5-25
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pubmed:databankReference |
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pubmed:abstractText |
The N-formyl peptide chemoattractant receptor (fMLF-R) is a cell-surface, G-protein-coupled glycoprotein that mediates the directed locomotion of neutrophils upon binding N-formylated peptides. The fMLF-R is encoded primarily by a 1.6-kb mRNA in differentiated HL-60 and U937 cells, although larger less abundant transcripts are present. To study the origin of different fMLF-R transcripts, the genetic linkage of chemotactic receptor genes, and the regulation of fMLF-R gene expression, we determined the copy number, chromosomal location, structural organization, and 5'-flanking sequence of the human fMLF-R gene. BamHI restriction fragments derived from a human fMLF-R genomic cosmid clone were isolated, subcloned, and sequenced. These data indicate that the fMLF-R structural gene is approximately 7.5 kb in length and is comprised of two exons separated by an approximately 5.0-kb intron. The first exon encodes 66 bp of the 5'-untranslated sequence, while exon 2 encodes the coding and 3'-untranslated sequences. The genomic organization of the fMLF-R gene is similar to that of the adrenergic beta-1 and beta-2 G-protein-coupled receptor genes in that the coding sequence is contained in a single exon. The different 3'-untranslated sequences observed in fMLF-R cDNA clones are contiguous in the genomic structure, thereby indicating that these clones are derived in part by alternative polyadenylation. Southern blot analysis using human X hamster somatic cell hybrids and in situ hybridization indicated that the h-fMLF-R gene is located on chromosome 19q13.3. Primer extension experiments using dbcAMP-differentiated U937 RNA indicated a single transcriptional initiation site. Sequence analysis 5' of the transcriptional initiation site indicated possible cis-acting motifs that may regulate fMLF-R gene expression. These included AP-1 and CK-2 consensus sequences that bind nuclear factors of the Fos/Jun family and NF-GMb, respectively.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/N-Formylmethionine...,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Poly A,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Formyl Peptide,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-2960
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4168-74
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7682842-Alternative Splicing,
pubmed-meshheading:7682842-Base Sequence,
pubmed-meshheading:7682842-Blotting, Northern,
pubmed-meshheading:7682842-Blotting, Southern,
pubmed-meshheading:7682842-Chromosome Banding,
pubmed-meshheading:7682842-Chromosome Mapping,
pubmed-meshheading:7682842-Chromosomes, Human, Pair 19,
pubmed-meshheading:7682842-Cloning, Molecular,
pubmed-meshheading:7682842-Cosmids,
pubmed-meshheading:7682842-DNA, Neoplasm,
pubmed-meshheading:7682842-Exons,
pubmed-meshheading:7682842-Humans,
pubmed-meshheading:7682842-In Situ Hybridization,
pubmed-meshheading:7682842-Introns,
pubmed-meshheading:7682842-Molecular Sequence Data,
pubmed-meshheading:7682842-N-Formylmethionine Leucyl-Phenylalanine,
pubmed-meshheading:7682842-Oligodeoxyribonucleotides,
pubmed-meshheading:7682842-Poly A,
pubmed-meshheading:7682842-RNA,
pubmed-meshheading:7682842-RNA, Messenger,
pubmed-meshheading:7682842-RNA, Neoplasm,
pubmed-meshheading:7682842-Receptors, Formyl Peptide,
pubmed-meshheading:7682842-Receptors, Immunologic,
pubmed-meshheading:7682842-Transcription, Genetic,
pubmed-meshheading:7682842-Tumor Cells, Cultured
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pubmed:year |
1993
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pubmed:articleTitle |
Structure, 5'-flanking sequence, and chromosome location of the human N-formyl peptide receptor gene. A single-copy gene comprised of two exons on chromosome 19q.13.3 that yields two distinct transcripts by alternative polyadenylation.
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pubmed:affiliation |
Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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