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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1993-5-27
|
| pubmed:abstractText |
The detection of antibody to the hepatitis C virus C100-3 antigen from the nonstructural region (NS3/NS4) of the viral genome was the first useful marker developed to detect past or potentially active infection with the hepatitis C virus. A systematic epitope survey of the nonstructural region has uncovered other immunogenic antigens. In order to assess the possible diagnostic utility of these antigens, their reactivity against a limited panel of sera from patients with chronic liver disease due to hepatitis C virus and other etiologies was tested. Antibody assays were performed using an immunoblot plaque assay and an enzyme-linked immunosorbent assay (ELISA). In a study of 16 C100-3-reactive individuals, all 16 patients were reactive using the plaque assay for the NS3 3' (409-1-1) and NS3 5' (C33u). In this same group of patients, antibodies by ELISA were reactive to NS3 3' in 12 of 16 patients (75%), NS3 5' in 15 of 16 patients (93%), and a capsid antigen (NC450) in 14 of 16 patients. In a group of five patients who were diagnosed with cryptogenic liver disease (C100-3 negative), 4 of 5 patients were reactive for antibody to all of the above epitopes. In a survey of 23 patients with other forms of chronic liver disease (nonviral liver disease, hepatitis B, alcoholic liver disease, cholestatic liver disease, and autoimmune hepatitis), only 1 of 23 patients was reactive for antibody to the C100-3 and 4 of 23 patients were reactive for antibodies to structural and nonstructural regions of the virus.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/C100 protein, hepatitis C virus,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis C Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Nonstructural Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0882-8245
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
6
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
N
|
| pubmed:pagination |
49-54
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7682814-Acute Disease,
pubmed-meshheading:7682814-Adult,
pubmed-meshheading:7682814-Amino Acid Sequence,
pubmed-meshheading:7682814-Antigens, Viral,
pubmed-meshheading:7682814-Base Sequence,
pubmed-meshheading:7682814-Biological Markers,
pubmed-meshheading:7682814-Capsid,
pubmed-meshheading:7682814-Child,
pubmed-meshheading:7682814-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:7682814-Epitopes,
pubmed-meshheading:7682814-Female,
pubmed-meshheading:7682814-Hepacivirus,
pubmed-meshheading:7682814-Hepatitis Antibodies,
pubmed-meshheading:7682814-Hepatitis C,
pubmed-meshheading:7682814-Hepatitis C Antibodies,
pubmed-meshheading:7682814-Humans,
pubmed-meshheading:7682814-Immunoblotting,
pubmed-meshheading:7682814-Liver Diseases,
pubmed-meshheading:7682814-Male,
pubmed-meshheading:7682814-Molecular Sequence Data,
pubmed-meshheading:7682814-Polymerase Chain Reaction,
pubmed-meshheading:7682814-Sexual Partners,
pubmed-meshheading:7682814-Viral Nonstructural Proteins,
pubmed-meshheading:7682814-Viral Plaque Assay
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Variation in antibody reactivity to the hepatitis C virus by comparative immunoscreening and enzyme immunoassay.
|
| pubmed:affiliation |
California Pacific Medical Center, San Francisco.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|