Linked Life Data

7682439

Source:http://linkedlifedata.com/resource/pubmed/id/7682439

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
1993-5-17
pubmed:abstractText
The immunological properties of alpha(2-->8) polysialic acid have been rationalized in terms of the presence of an epitope situated on a unique extended helical segment (n approximately 9) of the polymer. The critical importance of the carboxylate group to the stability of the extended helical epitope can be ascertained from NMR spectrocopic studies and potential energy calculations on the carboxyl reduced alpha(2-->8) polysialic acid. These studies indicate that the extended helix (n approximately 9) is not stabilized in the reduced polymer and that the majority of conformers can only have helical parameters with n = 2 and 3. This result is also consistent with the fact that the reduced alpha(2-->8) polysialic acid, contrary to its acidic counterpart, exhibits conventional immunological properties. Only five to six reduced oligomers are required to inhibit the binding of the reduced polysialic acid to its homologous antiserum. NMR spectroscopic analysis and potential energy calculations on the N-propionyl, N-butanoyl, N-isobutanoyl, N-pentanoyl, N-hexanoyl, and N-glycolyl derivatives of alpha(2-->8) polysialic acid indicate that, despite the bulk of some of these substituents, they did not disrupt the extended helical conformer. The presence of the extended helical epitope in some of these N-acyl derivatives has also been confirmed from immunological data.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4007-13
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Comparison of the conformation of the epitope of alpha(2-->8) polysialic acid with its reduced and N-acyl derivatives.
pubmed:affiliation
Institute for Biological Sciences, National Research Council of Canada, Ottawa.
pubmed:publicationType
Journal Article, Comparative Study