Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-5-13
pubmed:abstractText
E-selectin is an inducible endothelial cell adhesion molecule for neutrophils which functions as a Ca(2+)-dependent lectin. Using a recombinant, antibody-like form of mouse E-selectin, we have searched for glycoprotein ligands on mouse neutrophils and the neutrophil progenitor cell line 32D cl 3. We have identified a 150-kD glycoprotein as the only protein which could be affinity-isolated with soluble E-selectin from [35S]methionine/[35S]cysteine-labeled 32D cl 3 cells. Binding of this protein was strictly Ca(2+)-dependent, was blocked by a cell adhesion-blocking mAb against mouse E-selectin, and required the presence of sialic acid on the 150-kD ligand. This glycoprotein was also affinity-isolated from mature neutrophils, in addition to a minor component at 250 kD, but could not be isolated from several other non-myeloid cell lines. The 150-kD glycoprotein was the only protein from 32D cl 3 cells, which was detectable by silver-staining after a one-step affinity-isolation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1367388, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1371678, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1372439, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1374233, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1374413, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1376638, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1378017, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1378449, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1378450, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1378846, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1382077, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1382586, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1384480, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1623209, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1688580, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1689464, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1701274, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1701275, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1704009, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1705667, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1712483, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1712790, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1713515, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1714447, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1716182, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1717159, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1718051, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1719556, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1721027, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1722400, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-1986307, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-2040648, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-2190992, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-2438328, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-2460470, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-2466335, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-2472431, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-2509939, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-2646713, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-2647302, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-2668305, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-2827173, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-3004743, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-3290208, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-3584977, http://linkedlifedata.com/resource/pubmed/commentcorrection/7682218-6866086
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
121
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
449-59
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Identification of a glycoprotein ligand for E-selectin on mouse myeloid cells.
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