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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1993-5-4
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pubmed:databankReference |
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pubmed:abstractText |
A 65-kDa protein and a 10-kDa protein are two of the more strongly immunoreactive components of Mycobacterium tuberculosis, the causative agent of tuberculosis. The 65-kDa antigen has homology with members of the GroEL or chaperonin-60 (Cpn60) family of heat shock proteins. The 10-kDa antigen has homology with the GroES or chaperonin-10 family of heat shock proteins. These two proteins are encoded by separate genes in M. tuberculosis. The studies reported here reveal that M. tuberculosis contains a second Cpn60 homolog located 98 bp downstream of the 10-kDa antigen gene. The second Cpn60 homolog (Cpn60-1) displays 61% amino acid sequence identity with the 65-kDa antigen (Cpn60-2) and 53% and 41% identity with the Escherichia coli GroEL protein and the human P60 protein, respectively. Primer-extension analysis revealed that transcription starts 29 bp upstream of the translation start of the Cpn60-1 homolog and protein purification studies indicate that the cpn60-1 gene is expressed as an approximately 60-kDa polypeptide.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7681982-1354834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7681982-1371791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7681982-1675978,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7681982-1695022,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7681982-6546423
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2608-12
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7681982-Antigens, Bacterial,
pubmed-meshheading:7681982-Bacterial Proteins,
pubmed-meshheading:7681982-Base Sequence,
pubmed-meshheading:7681982-Chaperonin 60,
pubmed-meshheading:7681982-Chromatography, Affinity,
pubmed-meshheading:7681982-DNA, Bacterial,
pubmed-meshheading:7681982-Gene Library,
pubmed-meshheading:7681982-Genes, Bacterial,
pubmed-meshheading:7681982-Heat-Shock Proteins,
pubmed-meshheading:7681982-Molecular Sequence Data,
pubmed-meshheading:7681982-Molecular Weight,
pubmed-meshheading:7681982-Mycobacterium bovis,
pubmed-meshheading:7681982-Mycobacterium tuberculosis,
pubmed-meshheading:7681982-Oligodeoxyribonucleotides,
pubmed-meshheading:7681982-RNA, Bacterial,
pubmed-meshheading:7681982-Restriction Mapping,
pubmed-meshheading:7681982-Sequence Homology, Amino Acid
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pubmed:year |
1993
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pubmed:articleTitle |
Mycobacterium tuberculosis expresses two chaperonin-60 homologs.
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pubmed:affiliation |
Department of Medical Microbiology, St. George's Hospital Medical School, London, United Kingdom.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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