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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1993-5-5
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pubmed:abstractText |
Putative hepatitis C virus core sequence was amplified from a serum sample positive for anti-C-100-3 and expressed in Escherichia coli. Approximately 62 kDa fusion protein with maltose binding protein containing 20 kDa hepatitis C core protein was obtained. The antibody to this protein was detected in 53 of 54 (98%) sera from hepatitis C virus ribonucleic acid-positive patients including 40 sera positive for anti-C-100-3 and 13 sera negative for anti-C-100-3. The antibody was also detected in all of 12 patients with acute hepatitis C showing the earlier detectability of the antibody than anti-C-100-3. Thus, the protein expressed from the amplified hepatitis C core sequence by the polymerase chain reaction would be useful for the diagnosis of hepatitis C.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis C Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Maltose-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Core Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/maltose transport system, E coli
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0163-2116
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
626-30
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:7681745-ATP-Binding Cassette Transporters,
pubmed-meshheading:7681745-Acute Disease,
pubmed-meshheading:7681745-Bacterial Proteins,
pubmed-meshheading:7681745-Base Sequence,
pubmed-meshheading:7681745-Blotting, Western,
pubmed-meshheading:7681745-Carrier Proteins,
pubmed-meshheading:7681745-Chronic Disease,
pubmed-meshheading:7681745-Escherichia coli,
pubmed-meshheading:7681745-Escherichia coli Proteins,
pubmed-meshheading:7681745-Female,
pubmed-meshheading:7681745-Hepacivirus,
pubmed-meshheading:7681745-Hepatitis Antibodies,
pubmed-meshheading:7681745-Hepatitis C,
pubmed-meshheading:7681745-Hepatitis C Antibodies,
pubmed-meshheading:7681745-Humans,
pubmed-meshheading:7681745-Maltose-Binding Proteins,
pubmed-meshheading:7681745-Middle Aged,
pubmed-meshheading:7681745-Molecular Sequence Data,
pubmed-meshheading:7681745-Molecular Weight,
pubmed-meshheading:7681745-Monosaccharide Transport Proteins,
pubmed-meshheading:7681745-Polymerase Chain Reaction,
pubmed-meshheading:7681745-RNA, Viral,
pubmed-meshheading:7681745-Viral Core Proteins,
pubmed-meshheading:7681745-Viral Fusion Proteins
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pubmed:year |
1993
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pubmed:articleTitle |
Expression of hepatitis C virus core protein as a fusion protein with maltose binding protein. Detection of anti-hepatitis C core antibody by western blot.
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pubmed:affiliation |
First Department of Medicine, Chiba University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article
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