Linked Life Data

7681517

Source:http://linkedlifedata.com/resource/pubmed/id/7681517

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-4-27
pubmed:abstractText
A combined Golgi-impregnation/immunocytochemistry procedure was developed to identify the endogenous neurotransmitter content of morphologically characterized neurons. Golgi-impregnated retinal amacrine cells in the lizard Anolis carolinensis were characterized morphologically in thick resin sections. Cells of interest were remounted, resectioned at 1 micron thickness and subjected to a postembedding immunofluorescence procedure to visualize the amino acid neurotransmitters gamma-aminobutyric acid (GABA) or glycine. Double-labeled cells were identified by opaque Golgi deposits in the cytoplasm under bright-field illumination and nuclear immunofluorescence under ultraviolet illumination. Twenty-seven Golgi-impregnated amacrine cells, exhibiting morphological features of GABA-immunoreactive (GABA-IR) cells, were tested for GABA-IR; 21 showed double labeling. Glycine-IR amacrine cells also were identified using the Golgi/immunocytochemistry procedure. This double-labeling procedure allows rapid assessment of endogenous neurotransmitter content in large samples of morphologically characterized neurons.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
41-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Immunofluorescent identification of endogenous neurotransmitter content in Golgi-impregnated neurons.
pubmed:affiliation
SUNY-Stony Brook, Department of Neurobiology and Behavior 11794-5230.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.