Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6415
|
| pubmed:dateCreated |
1993-4-6
|
| pubmed:abstractText |
Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system in which a restricted cellular immune response has been observed. In order to establish whether such T cell responses are likely to be antigen-specific particularly with regard to myelin basic protein (MBP), we analysed T-cell receptor (TCR) gene rearrangements directly from MS brain plaques, using the polymerase chain reaction on reverse transcribed messenger RNA, and compared these with TCR of previously described MBP-specific T cell clones from MS and the rat model experimental allergic encephalomyelitis. Rearranged V beta 5.2 genes were detected in the brains of all patients who were HLA DRB1*1501, DQA1*0102, DQB1*0602, DPB1*0401. The V beta 5.2-D beta-J beta sequences in these MS brain plaques revealed five motifs. One of the common motifs was identical to that described for the VDJ region of a V beta 5.2 T-cell clone. This clone was from an MS patient who was HLA DRB1*1501, DQB1*0602, DPB1*0401, and it was cytotoxic towards targets containing the MBP peptide 89-106 (ref. 1). The deduced amino-acid sequence of this VDJ rearrangement, Leu-Arg-Gly, has also been described in rat T cells cloned from experimental allergic encephalomyelitis lesions, which are specific for MBP peptide 87-99 (ref. 2). VDJ sequences with specificity for this MBP epitope constitute a large fraction (40%) of the TCR V beta 5.2 N(D)N rearrangements in MS lesions. The capacity of rat T cells with these VDJ sequences to cause experimental allergic encephalomyelitis and the prevalence of such sequences in demyelinated human lesions indicate that T cells with this rearranged TCR may be critical in MS.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-D Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Myelin Basic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0028-0836
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
4
|
| pubmed:volume |
362
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
N
|
| pubmed:pagination |
68-70
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7680433-Amino Acid Sequence,
pubmed-meshheading:7680433-Animals,
pubmed-meshheading:7680433-Antigens, CD3,
pubmed-meshheading:7680433-Base Sequence,
pubmed-meshheading:7680433-Brain,
pubmed-meshheading:7680433-Cloning, Molecular,
pubmed-meshheading:7680433-Encephalomyelitis, Autoimmune, Experimental,
pubmed-meshheading:7680433-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:7680433-HLA-D Antigens,
pubmed-meshheading:7680433-Humans,
pubmed-meshheading:7680433-Molecular Sequence Data,
pubmed-meshheading:7680433-Multiple Sclerosis,
pubmed-meshheading:7680433-Myelin Basic Proteins,
pubmed-meshheading:7680433-Oligodeoxyribonucleotides,
pubmed-meshheading:7680433-Phenotype,
pubmed-meshheading:7680433-Polymerase Chain Reaction,
pubmed-meshheading:7680433-RNA, Messenger,
pubmed-meshheading:7680433-Rats,
pubmed-meshheading:7680433-Spinal Cord,
pubmed-meshheading:7680433-T-Lymphocytes
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Selection for T-cell receptor V beta-D beta-J beta gene rearrangements with specificity for a myelin basic protein peptide in brain lesions of multiple sclerosis.
|
| pubmed:affiliation |
Department of Neurology and Neurological Science, Stanford University School of Medicine, California 94305-5235.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|