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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1993-3-26
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pubmed:databankReference | |
pubmed:abstractText |
Complementary DNA encoding a rat kidney chloride channel (CIC-K1) was isolated by a polymerase chain reaction (PCR) cloning strategy. We designed degenerate primers, based on the regions where previously cloned chloride channels (CIC-0, -1, and -2) possess significant amino acid identity, and performed reverse transcription PCR with whole kidney mRNA. The 686-amino acid protein encoded by CIC-K1 is about 40% identical to the previously cloned chloride channels and has a similar hydropathy profile. Expression of CIC-K1 in Xenopus oocytes induced Cl- currents that activate instantaneously upon hyperpolarization and depolarization, and displayed a slightly outwardly rectifying current-voltage relationship. The message for CIC-K1 was 2.4 kilobases and was found predominantly in kidney, especially in the inner medulla. Reverse transcription PCR technique using micro-dissected nephron segments revealed that the main site of expression in kidney was the thin ascending limb of Henle's loop, which has the highest Cl- permeability among the nephron segments and is thought to be involved in a counter-current system for urine concentration in the inner medulla. The abundance of CIC-K1 mRNA in kidney increased about 4-fold as rats became dehydrated by deprivation of water for 5 days. The site of expression and the regulation by dehydration suggest that CIC-K1 function may be important in urinary concentrating mechanisms.
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pubmed:commentsCorrections | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloride Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Single-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Water
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
268
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3821-4
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:7680033-Amino Acid Sequence,
pubmed-meshheading:7680033-Animals,
pubmed-meshheading:7680033-Base Sequence,
pubmed-meshheading:7680033-Chloride Channels,
pubmed-meshheading:7680033-Chlorides,
pubmed-meshheading:7680033-Cloning, Molecular,
pubmed-meshheading:7680033-DNA, Single-Stranded,
pubmed-meshheading:7680033-Gene Expression Regulation,
pubmed-meshheading:7680033-Ion Channels,
pubmed-meshheading:7680033-Kidney Medulla,
pubmed-meshheading:7680033-Membrane Proteins,
pubmed-meshheading:7680033-Molecular Sequence Data,
pubmed-meshheading:7680033-RNA, Messenger,
pubmed-meshheading:7680033-Sequence Homology, Amino Acid,
pubmed-meshheading:7680033-Water,
pubmed-meshheading:7680033-Xenopus
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pubmed:year |
1993
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pubmed:articleTitle |
Molecular cloning of a chloride channel that is regulated by dehydration and expressed predominantly in kidney medulla.
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pubmed:affiliation |
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
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pubmed:publicationType |
Journal Article
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