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pubmed-article:7680023pubmed:abstractTextThe goal of this study was to develop the first vital assay system for in vitro analysis of phagocytosis of rod outer segments (ROS) by normal retinal pigment epithelial (RPE) cells and for study of the phagocytic defect in RPE of the Royal College of Surgeons (RCS) rat with inherited retinal degeneration. Required features included ability to directly visualize and quantitate the phagocytic process in living RPE cultures, and capability for subsequent quantitative analysis after fixation of the cells at any chosen time after incubation with ROS.lld:pubmed
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pubmed-article:7680023pubmed:year1993lld:pubmed
pubmed-article:7680023pubmed:articleTitleDouble fluorescent vital assay of phagocytosis by cultured retinal pigment epithelial cells.lld:pubmed
pubmed-article:7680023pubmed:affiliationDepartment of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Florida 33101.lld:pubmed
pubmed-article:7680023pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:7680023pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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