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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-3-29
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pubmed:abstractText |
The goal of this study was to develop the first vital assay system for in vitro analysis of phagocytosis of rod outer segments (ROS) by normal retinal pigment epithelial (RPE) cells and for study of the phagocytic defect in RPE of the Royal College of Surgeons (RCS) rat with inherited retinal degeneration. Required features included ability to directly visualize and quantitate the phagocytic process in living RPE cultures, and capability for subsequent quantitative analysis after fixation of the cells at any chosen time after incubation with ROS.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0146-0404
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
317-26
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pubmed:dateRevised |
2010-3-24
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pubmed:meshHeading |
pubmed-meshheading:7680023-Animals,
pubmed-meshheading:7680023-Cells, Cultured,
pubmed-meshheading:7680023-Disease Models, Animal,
pubmed-meshheading:7680023-Fluorescein-5-isothiocyanate,
pubmed-meshheading:7680023-Fluorescent Antibody Technique,
pubmed-meshheading:7680023-Lysosomes,
pubmed-meshheading:7680023-Microscopy, Fluorescence,
pubmed-meshheading:7680023-Phagocytosis,
pubmed-meshheading:7680023-Phagosomes,
pubmed-meshheading:7680023-Pigment Epithelium of Eye,
pubmed-meshheading:7680023-Rats,
pubmed-meshheading:7680023-Rats, Mutant Strains,
pubmed-meshheading:7680023-Retinal Degeneration,
pubmed-meshheading:7680023-Rhodamines,
pubmed-meshheading:7680023-Rod Cell Outer Segment,
pubmed-meshheading:7680023-Staining and Labeling
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pubmed:year |
1993
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pubmed:articleTitle |
Double fluorescent vital assay of phagocytosis by cultured retinal pigment epithelial cells.
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pubmed:affiliation |
Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Florida 33101.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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