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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1993-3-17
|
| pubmed:abstractText |
To clarify whether hepatocellular carcinoma (HCC) may produce and secrete endothelin (ET), we measured plasma levels of ET-1 and big ET-1, a precursor form of ET-1, in 30 patients with HCC. When compared to normal subjects, a substantial number of patients had elevated plasma ET-1 and big ET-1 levels, determined by specific enzyme immunoassays. The mean (+/- SD) plasma concentrations of ET-1 (1.7 +/- 0.9 pmol/L) and big ET-1 (6.1 +/- 4.8 pmol/L) in patients' group were significantly (P < 0.01) higher than those (1.0 +/- 0.3 and 2.0 +/- 0.8 pmol/L, respectively) in control group. There was a significant positive correlation between plasma big ET-1 and alpha-fetoprotein (r = 0.77, P < 0.01). Some of the 29 patients with liver cirrhosis also had modestly elevated plasma big ET-1 levels. The mean (+/- SD) plasma big ET-1 concentration (3.1 +/- 0.9 pmol/L) in patients with liver cirrhosis was significantly (P < 0.01) higher than that in control group, although there was no significant difference between the mean plasma ET-1 levels of both groups. Raised plasma big ET-1 and, less markedly, ET-1 levels in patients with HCC decreased after successful transcatheter arterial embolization concomitantly with a reduction in tumor sizes and a decrease in plasma alpha-fetoprotein levels. In six patients, an arteriovenous difference in ET-1 and big ET-1 levels across the tumor bed with a higher concentration in the venous circulation was found. Reverse-phase high performance liquid chromatography revealed that major portions of immunoreactive ET-1 and big ET-1 in hepatic venous plasma coeluted with synthetic ET-1 and big ET-1, respectively. Immunohistochemistry of HCC tissues from two patients demonstrated HCC cells positive for ET-1 and big ET-1, whereas no ET immunoreactivity was found in adjacent nontumorous hepatocytes. We conclude from these results that ET is produced by and released from a substantial number of HCC, which may stimulate proliferation of carcinoma cells as an autocrine or paracrine growth factor.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0021-972X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
76
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
378-83
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7679399-Adult,
pubmed-meshheading:7679399-Aged,
pubmed-meshheading:7679399-Arteries,
pubmed-meshheading:7679399-Carcinoma, Hepatocellular,
pubmed-meshheading:7679399-Chromatography, High Pressure Liquid,
pubmed-meshheading:7679399-Embolization, Therapeutic,
pubmed-meshheading:7679399-Endothelin-1,
pubmed-meshheading:7679399-Endothelins,
pubmed-meshheading:7679399-Female,
pubmed-meshheading:7679399-Humans,
pubmed-meshheading:7679399-Immunoenzyme Techniques,
pubmed-meshheading:7679399-Liver Cirrhosis,
pubmed-meshheading:7679399-Liver Neoplasms,
pubmed-meshheading:7679399-Male,
pubmed-meshheading:7679399-Middle Aged,
pubmed-meshheading:7679399-Protein Precursors,
pubmed-meshheading:7679399-Veins,
pubmed-meshheading:7679399-alpha-Fetoproteins
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Production and secretion of endothelin by hepatocellular carcinoma.
|
| pubmed:affiliation |
Fourth Department of Medicine, Teikyo University School of Medicine, Kawasaki, Japan.
|
| pubmed:publicationType |
Journal Article
|