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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1993-3-17
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pubmed:abstractText |
The expression of the proto-oncogene c-fos is increased in neuronal cells by a number of stimuli and the usefulness of this gene as a marker of neuronal activity has been demonstrated. The temporal and spatial expression of c-fos mRNA following the induction of a unilateral cortical lesion have been investigated in the rat brain by Northern blot analysis and in situ hybridization histochemistry. It was observed that the lesion evoked a rapid increase (20-fold) in the content of c-fos mRNA in the ipsilateral cortex, whereas in the contralateral cortex c-fos mRNA expression was more modest (7-fold). In the whole hippocampus a large and very rapid increase (17-fold) of c-fos mRNA expression was detected. The effect of a cortical lesion on Ca2+ uptake and membrane potential was also investigated. Using synaptosomes as a model system, we have provided evidence that Ca2+ entry via membrane depolarization increases in coordination with c-fos gene expression in neuronal cells. The principal conclusions from this study are that cortical lesions induce transient expression of the c-fos gene in specific neuronal cells of the rat brain.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-8993
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
601
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pubmed:geneSymbol |
c-fos
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
230-40
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7679308-Animals,
pubmed-meshheading:7679308-Antineoplastic Agents,
pubmed-meshheading:7679308-Blotting, Northern,
pubmed-meshheading:7679308-Brain Chemistry,
pubmed-meshheading:7679308-Calcium,
pubmed-meshheading:7679308-Calcium Radioisotopes,
pubmed-meshheading:7679308-Cerebral Cortex,
pubmed-meshheading:7679308-Gene Expression,
pubmed-meshheading:7679308-Genes, fos,
pubmed-meshheading:7679308-In Situ Hybridization,
pubmed-meshheading:7679308-Male,
pubmed-meshheading:7679308-Membrane Potentials,
pubmed-meshheading:7679308-Onium Compounds,
pubmed-meshheading:7679308-Organophosphorus Compounds,
pubmed-meshheading:7679308-RNA,
pubmed-meshheading:7679308-RNA, Messenger,
pubmed-meshheading:7679308-Rats,
pubmed-meshheading:7679308-Signal Transduction,
pubmed-meshheading:7679308-Synaptosomes,
pubmed-meshheading:7679308-Transcription, Genetic
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pubmed:year |
1993
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pubmed:articleTitle |
Temporal and spatial preferences of c-fos mRNA expression in the rat brain following cortical lesion.
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pubmed:affiliation |
Department of Neurobiology and Immunology, University of Belgrade, Yugoslavia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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