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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1993-2-23
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pubmed:abstractText |
We have demonstrated that human fibroblasts can release O2-. radicals by an NADPH oxidase system that appears to be functionally similar to the phagocytic system. Further analysis of these systems, however, with respect to the low-potential b-type cytochromes involved suggests that these two O2-.-generating systems are not structurally identical. Immunoblot analysis of fibroblast membranes with six different antibodies directed against both subunits of human neutrophil cytochrome b-558 indicated that the b-type cytochrome molecules involved in these systems were not identical. None of these anti-(neutrophil cytochrome b) antibodies recognized a similar cytochrome in fibroblast membranes, suggesting that the two cytochrome species are immunologically distinct. In addition, fibroblasts obtained from a patient suffering from X-linked chronic granulomatous disease (CGD) had a normal cytochrome b-558 content compared with control fibroblast membranes, whereas the cytochrome b-558 concentration in polymorphonuclear leucocytes (PMNs) from this patient was decreased to 10% of that found in PMNs from healthy controls. Likewise, the stimulated O2-. release in PMNs from this patient was less than 10% of that in control PMNs, whereas the fibroblasts showed stimulated O2-.-release rates that were indistinguishable from those of fibroblasts obtained from healthy persons. Since the genetic mutation responsible for this type of CGD results in the absence of cytochrome b-558 in PMNs, fibroblasts should be affected in the same way if both cytochrome species were identical. These results suggest therefore that the low-potential b-type cytochromes in PMNs and fibroblasts are structurally and genetically distinct.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-115327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-1559974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-1657945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-1847135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-1850240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-1851438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-1858784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-1963784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-1985107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-2155923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-2161041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-2312727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-2391431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-2469497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-2509942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-2656760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-2834275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-289386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-2985050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-3021215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-3179440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-3368442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-3800872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-4943714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-5389100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-6101356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-6119486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-6254418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-6282132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-6386073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-723935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-7306004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-732578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678734-845166
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
289 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
481-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7678734-Antibodies,
pubmed-meshheading:7678734-Blotting, Western,
pubmed-meshheading:7678734-Cell Line,
pubmed-meshheading:7678734-Cell Membrane,
pubmed-meshheading:7678734-Cytochrome b Group,
pubmed-meshheading:7678734-Epitopes,
pubmed-meshheading:7678734-Fanconi Anemia,
pubmed-meshheading:7678734-Fibroblasts,
pubmed-meshheading:7678734-Humans,
pubmed-meshheading:7678734-Kinetics,
pubmed-meshheading:7678734-Macromolecular Substances,
pubmed-meshheading:7678734-NADH, NADPH Oxidoreductases,
pubmed-meshheading:7678734-NADPH Oxidase,
pubmed-meshheading:7678734-Neutrophils,
pubmed-meshheading:7678734-Superoxides
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pubmed:year |
1993
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pubmed:articleTitle |
The cytochrome b-558 molecules involved in the fibroblast and polymorphonuclear leucocyte superoxide-generating NADPH oxidase systems are structurally and genetically distinct.
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pubmed:affiliation |
Chemisches Institute, Tierärztliche Hochschule, Hannover, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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