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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1993-2-24
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pubmed:abstractText |
To delineate how gene rearrangement influences the expressed human gamma delta T cell repertoire, we generated T cell receptor gamma (TCR gamma) V domain-specific cDNA libraries from the peripheral lymphocytes of eight donors and sequenced a total of 232 TCR gamma gene transcripts. The libraries consisted of both in-frame and out-of-frame rearranged TCR gamma genes. The in-frame TCR gamma gene transcripts were used to determine the diversity of functional T cells, whereas the out-of-frame transcripts, primarily derived from alpha beta T cells, were used to assess the frequencies of TCR V gamma-J gamma rearrangements in progenitor T lymphocytes. The results showed that both sets of transcripts exhibited strikingly restricted V gamma-J gamma combinations. Only 11 of 40 potential V gamma-J gamma rearrangements were common ( > or = 3% of total). The pattern of gene usage in the functional and nonfunctional transcripts was similar and did not differ markedly among donors. The only exception was the predominance of V gamma 9-JP in potentially functional transcripts from seven of eight individuals. These results show that V gamma-J gamma rearrangement is nonrandom and suggest that the diversity of TCR gamma genes in the functional gamma delta T cell repertoire partly depends upon preferentially rearranged V gamma-J gamma gene combinations. However, the expansion of V gamma 9/V gamma 2 T cells in adult peripheral blood can only be explained by antigenic selection of relatively rare V gamma 9-JP recombinants.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-1310453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-1310710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-1318903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-1700054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-1711558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-1827824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-1832874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-1978758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-1989888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2146317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2164429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2167345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2185330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2326267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2377230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2450164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2463672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2477444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2501444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2538552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2583098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2820713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2938743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2949984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2951442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2960549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2963227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-2967764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-3055853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-3263423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-3263574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-3494522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678601-7302589
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
171-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7678601-Adult,
pubmed-meshheading:7678601-Base Sequence,
pubmed-meshheading:7678601-Chromosomes, Human, Pair 7,
pubmed-meshheading:7678601-Cloning, Molecular,
pubmed-meshheading:7678601-Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor,
pubmed-meshheading:7678601-Humans,
pubmed-meshheading:7678601-Middle Aged,
pubmed-meshheading:7678601-Molecular Sequence Data,
pubmed-meshheading:7678601-Oligodeoxyribonucleotides,
pubmed-meshheading:7678601-Oligonucleotides, Antisense,
pubmed-meshheading:7678601-Polymerase Chain Reaction,
pubmed-meshheading:7678601-Protein Biosynthesis,
pubmed-meshheading:7678601-RNA,
pubmed-meshheading:7678601-Reading Frames,
pubmed-meshheading:7678601-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:7678601-Recombination, Genetic,
pubmed-meshheading:7678601-T-Lymphocytes,
pubmed-meshheading:7678601-Transcription, Genetic
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pubmed:year |
1993
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pubmed:articleTitle |
Regulation of the mature human T cell receptor gamma repertoire by biased V-J gene rearrangement.
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pubmed:affiliation |
Department of Medicine, University of California, San Diego, La Jolla 92093.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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