Linked Life Data

7678589

Source:http://linkedlifedata.com/resource/pubmed/id/7678589

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-2-22
pubmed:abstractText
Sixty-two rabbit anti-lipid A serum samples were compared with respect to the immunogens used (synthetic lipid A and partial structures, natural lipid A, or acid-treated bacteria). Immunoglobulin (Ig) type-specific differences in rabbit response between liposomal membrane-embedded (LME) and other lipid A immunogens were found: LME lipid A elicited predominantly IgM antibodies. Previous findings of equally good immune responses to synthetic lipid A and acid-treated bacteria (L. Brade, E.T. Rietschel, S. Kusumoto, T. Shiba, and H. Brade, Infect. Immun. 51:110-114, 1986, and L. Brade, E.T. Rietschel, S. Kusumoto, T. Shiba, and H. Brade, Prog. Clin. Biol. Res. 231:75-97, 1987) turned out to be restricted to complement-fixing antibodies; IgG titers of sera against free lipid A (whether synthetic or natural) were significantly lower than those raised with bacteria. The results indicated an increase in IgG content of sera from LME lipid A over other free lipid A immunogens to acid-treated bacteria. These data underline the importance of the physicochemical environment for the immunogenicity of lipid A. As a second objective, the presence of various lipid A antibody specificities was tested with synthetic lipid A antigens. Antibodies to monophosphoryl lipid A were detected only in sera raised with monophosphoryl immunogens. Reactivity with monosaccharide partial structures of lipid A was found both in sera against monophosphoryl lipid A and in 60% of sera against bisphosphoryl lipid A. In the former, monosaccharide reactivity was of a magnitude similar to that of reactivity with lipid A; in sera against bisphosphoryl lipid A, it was lower. No reactivity or only marginal reactivity was found with phosphate-free lipid A, thus emphasizing the role of phosphate substitution for the lipid A epitopes recognized.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7678589-1089729, http://linkedlifedata.com/resource/pubmed/commentcorrection/7678589-115918, http://linkedlifedata.com/resource/pubmed/commentcorrection/7678589-1375194, http://linkedlifedata.com/resource/pubmed/commentcorrection/7678589-1431148, http://linkedlifedata.com/resource/pubmed/commentcorrection/7678589-2183560, http://linkedlifedata.com/resource/pubmed/commentcorrection/7678589-2416687, http://linkedlifedata.com/resource/pubmed/commentcorrection/7678589-2438706, http://linkedlifedata.com/resource/pubmed/commentcorrection/7678589-2581900, http://linkedlifedata.com/resource/pubmed/commentcorrection/7678589-2689350, http://linkedlifedata.com/resource/pubmed/commentcorrection/7678589-334973, http://linkedlifedata.com/resource/pubmed/commentcorrection/7678589-3666956, http://linkedlifedata.com/resource/pubmed/commentcorrection/7678589-448082, http://linkedlifedata.com/resource/pubmed/commentcorrection/7678589-4944989
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
680-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Immune response of rabbits to lipid A: influence of immunogen preparation and distribution of various lipid A specificities.
pubmed:affiliation
Division of Biochemical Microbiology, Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Germany.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't