Linked Life Data

7674805

Source:http://linkedlifedata.com/resource/pubmed/id/7674805

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1995-10-19
pubmed:abstractText
To evaluate the regulatory action of protein kinase C (PKC) on airway beta-adrenergic function, the relaxant effects of isoproterenol (ISO) and 8 bromo-cyclic AMP (BrcAMP) were examined in tracheal smooth muscle (TSM) segments half-maximally contracted with acetylcholine in the absence (control) and presence of PKC activation with the phorbol ester, 12-deoxyphorbol 13-isobutyrate (DPB). Relative to control tissues, TSM treated with 0.1 microM DPB depicted significantly enhanced maximal relaxation and sensitivity to ISO but not to BrcAMP. The enhancing effect of DPB on ISO responsiveness was completely inhibited in the presence of the PKC antagonist H-7. Inhibition of the Na(+)-K+ pump with either ouabain or K(+)-free buffer diminished the TSM relaxant response to ISO but not to BrcAMP. Inhibition of the Na(+)-K+ pump also ablated the DPB-induced potentiation of beta-adrenoceptor responsiveness. Collectively, these data demonstrate that: 1) PKC activation enhances TSM relaxant responsiveness to beta-adrenoceptor stimulation; 2) inhibition of the airway Na(+)-K+ pump markedly blunts the relaxant response to beta-adrenoceptor stimulation; and 3) inhibition of the Na(+)-K+ pump abolishes the above potentiating effect of DPB on beta-adrenoceptor-mediated relaxation of rabbit TSM. Thus, the above findings provide new evidence that PKC activation enhances the airway relaxant response to beta-adrenoceptor stimulation, and that the latter effect is dependent on potentiated stimulation of the airway electrogenic Na(+)-K+ pump.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0024-3205
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1163-73
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Mechanism of protein kinase C potentiation of airway beta-adrenergic relaxation.
pubmed:affiliation
Pediatric Pulmonary Division, Univ. of Connecticut School of Medicine, Farmington 06030, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.