| pubmed-article:7671108 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7671108 | lifeskim:mentions | umls-concept:C0206679 | lld:lifeskim |
| pubmed-article:7671108 | lifeskim:mentions | umls-concept:C0021311 | lld:lifeskim |
| pubmed-article:7671108 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:7671108 | lifeskim:mentions | umls-concept:C0027754 | lld:lifeskim |
| pubmed-article:7671108 | lifeskim:mentions | umls-concept:C0085262 | lld:lifeskim |
| pubmed-article:7671108 | lifeskim:mentions | umls-concept:C0027752 | lld:lifeskim |
| pubmed-article:7671108 | lifeskim:mentions | umls-concept:C0086022 | lld:lifeskim |
| pubmed-article:7671108 | lifeskim:mentions | umls-concept:C1705099 | lld:lifeskim |
| pubmed-article:7671108 | lifeskim:mentions | umls-concept:C0442335 | lld:lifeskim |
| pubmed-article:7671108 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:7671108 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:7671108 | pubmed:dateCreated | 1995-10-19 | lld:pubmed |
| pubmed-article:7671108 | pubmed:abstractText | We have developed an HSV-1 vector with mutations in the viral IE 3 and VP16 genes that expresses mouse beta nerve growth factor (NGF) from a latency associated transcript (LAT) promoter modified by insertion of a Rous sarcoma virus (RSV) enhancer. The backbone double mutant vector has reduced cytotoxicity compared with a single mutant deleted for IE 3 and is able to express the reporter luciferase gene in rat pheochromocytoma (PC12) cells at low but relatively stable levels in vitro. Intracellular NGF levels of approximately 3 pg per mg of cellular protein were detected in infected PC12 cells for at least 7 days after infection. Furthermore, infected PC12 cells exhibited differentiated morphology marked by neurite outgrowth similar to that found after exposure of PC12 cells to purified 2.5S beta NGF. A persistent level of 0.2 to 0.3 pM of the expressed NGF was detected in the culture media. The NGF-expressing vector also showed reduced cytotoxicity compared with that of the parental virus in infected PC12 cells. In PC12 cells that overexpress the human proto-oncogene bcl-2, the cytotoxicity of both viruses was significantly reduced. These studies demonstrate that the reduced cytotoxicity of the IE 3-VP16 double mutant virus and the increased duration of transgene expression from the RSV-modified LAT promoter permit terminal differentiation of PC12 cells after infection with an NGF-expressing HSV-1 vector. | lld:pubmed |
| pubmed-article:7671108 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7671108 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7671108 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7671108 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7671108 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7671108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7671108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7671108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7671108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7671108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7671108 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7671108 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:7671108 | pubmed:issn | 0969-7128 | lld:pubmed |
| pubmed-article:7671108 | pubmed:author | pubmed-author:FriedmannTT | lld:pubmed |
| pubmed-article:7671108 | pubmed:author | pubmed-author:JohnsonP APA | lld:pubmed |
| pubmed-article:7671108 | pubmed:author | pubmed-author:WangM JMJ | lld:pubmed |
| pubmed-article:7671108 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7671108 | pubmed:volume | 2 | lld:pubmed |
| pubmed-article:7671108 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7671108 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7671108 | pubmed:pagination | 323-35 | lld:pubmed |
| pubmed-article:7671108 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:7671108 | pubmed:meshHeading | pubmed-meshheading:7671108-... | lld:pubmed |
| pubmed-article:7671108 | pubmed:meshHeading | pubmed-meshheading:7671108-... | lld:pubmed |
| pubmed-article:7671108 | pubmed:meshHeading | pubmed-meshheading:7671108-... | lld:pubmed |
| pubmed-article:7671108 | pubmed:meshHeading | pubmed-meshheading:7671108-... | lld:pubmed |
| pubmed-article:7671108 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7671108 | pubmed:articleTitle | Differentiation of PC12 cells by infection with an HSV-1 vector expressing nerve growth factor. | lld:pubmed |
| pubmed-article:7671108 | pubmed:affiliation | Department of Pediatrics, University of California at San Diego, La Jolla 92093-0634, USA. | lld:pubmed |
| pubmed-article:7671108 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7671108 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7671108 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7671108 | lld:pubmed |
