Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1978-6-12
pubmed:abstractText
A previously described type virus stock (designated PP-1R), isolated by cocultivating baboon cells with mink cells transformed by Kirsten sarcoma virus (64J1), has been further cloned and characterized. End point-diluted stocks of PP-1R have been obtained that are free of focus-forming activity and lack both Kirsten sarcoma and primate type C viral sequences. Nucleic acid hybridization experiments show that the cloned virus (MiLV) is an endogenous, genetically transmitted virus of the mink (Mustela vison). MiLV replicates in canine, feline, and 64J1 mink cells but not in an untransformed mink cell line. Multiple viral gene copies can be detected in the DNA of normal mink cells in culture and in normal mink tissues; related endogenous viral genes are also detected in several related Mustela species. The virus codes for a p30 protein very closely related antigenically to that of feline leukemia virus but contains p15 and p12 proteins that are antigenically distinct. The mink cell line, Mv1Lu, and its Kirsten sarcoma-transformed derivatives, 64J1, express relatively low levels of type C viral RNA related to MiLV and normally do not produce detectable levels of MiLV p30 protein or complete, infectious viral particles. Infection of sarcoma virus-transformed mink cells with baboon type C virus, however, can augment the level of expression of endogenous mink viral RNA and can result in the synthesis and packaging of mink viral RNA and p30 antigen in extracellular virions. Since the Mv1Lu cell line and its tranformed derivatives have become widely used in studies of retroviruses, the possibility of activating endogenous mink viral genes should be considered by investigators working with these cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-14097352, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-165619, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-16592456, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-166200, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-167514, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-169043, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-170737, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-178897, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-179013, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-181138, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-187944, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-191826, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-194310, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4111969, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4131937, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4135772, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4287053, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4335524, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4357758, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4365323, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4366800, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4370309, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4371148, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4372406, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4372546, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4373709, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4505653, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4626657, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4633070, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4868544, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-4874239, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-49058, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-49122, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-49443, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-52892, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-52940, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-5758028, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-5793196, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-58071, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-5926742, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-59458, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-60495, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-62362, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-62848, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-62849, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-64624, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-66330, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-69718, http://linkedlifedata.com/resource/pubmed/commentcorrection/76684-844104
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
738-49
pubmed:dateRevised
2010-9-1
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Endogenous mink (Mustela vison) type C virus isolated from sarcoma virus-transformed mink cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.