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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1995-10-11
|
| pubmed:abstractText |
Immunocytochemistry, using antibodies against subunit c of mitochondrial ATP synthase, has been carried out in the ovine, canine, late infantile, and adult forms of ceroid-lipofuscinosis. Intensity of staining varied depending on the particular disease, species, fixation regime, and the antibody used. Differential staining of storage cytosomes in neurons of affected sheep and those in the late infantile patient suggested exposure of different epitopes. This was supported by the variable staining using two different antibodies in ovine, late infantile, and adult onset (Kufs) diseases. Immunostaining of muscle in the late infantile, and muscle and ear cartilage in affected sheep can assist diagnosis but positive results may depend on the age of the patient, at least in the latter species. In these tissues there was immunostaining of structures not identified by histochemical or fluorescence microscopy in addition to storage cytosomes that could be identified by these means. Poor or no immunostaining occurred with canine tissues. At the ultrastructural level, storage cytosomes but not other organelles stained with the immunogold method.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0148-7299
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
57
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
177-81
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7668326-Adult,
pubmed-meshheading:7668326-Animals,
pubmed-meshheading:7668326-Autopsy,
pubmed-meshheading:7668326-Biopsy,
pubmed-meshheading:7668326-Cartilage,
pubmed-meshheading:7668326-Cerebral Cortex,
pubmed-meshheading:7668326-Cytoplasmic Granules,
pubmed-meshheading:7668326-Dog Diseases,
pubmed-meshheading:7668326-Dogs,
pubmed-meshheading:7668326-Humans,
pubmed-meshheading:7668326-Immunohistochemistry,
pubmed-meshheading:7668326-Infant,
pubmed-meshheading:7668326-Macromolecular Substances,
pubmed-meshheading:7668326-Microscopy, Immunoelectron,
pubmed-meshheading:7668326-Mitochondria,
pubmed-meshheading:7668326-Mitochondria, Muscle,
pubmed-meshheading:7668326-Muscle, Skeletal,
pubmed-meshheading:7668326-Neuronal Ceroid-Lipofuscinoses,
pubmed-meshheading:7668326-Proton-Translocating ATPases,
pubmed-meshheading:7668326-Sheep,
pubmed-meshheading:7668326-Sheep Diseases
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Immunocytochemical studies in the ceroid-lipofuscinoses (Batten disease) using antibodies to subunit c of mitochondrial ATP synthase.
|
| pubmed:affiliation |
Department of Veterinary Pathology and Public Health, Massey University, Palmerston North, New Zealand.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|