Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
1995-10-12
pubmed:abstractText
An EPR "spectroscopic ruler" was developed using a series of alpha-helical polypeptides, each modified with two nitroxide spin labels. The EPR line broadening due to electron-electron dipolar interactions in the frozen state was determined using the Fourier deconvolution method. These dipolar spectra were then used to estimate the distances between the two nitroxides separated by 8-25 A. Results agreed well with a simple alpha-helical model. The standard deviation from the model system was 0.9 A in the range of 8-25 A. This technique is applicable to complex systems such as membrane receptors and channels, which are difficult to access with high-resolution NMR or x-ray crystallography, and is expected to be particularly useful for systems for which optical methods are hampered by the presence of light-interfering membranes or chromophores.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-1312255, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-1328890, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-1591245, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-1660187, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-1661030, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-2160734, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-262414, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-2748584, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-345054, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-5233469, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-789892, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-8218270, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-8218281, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-8382373, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-8428572, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-8469972, http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-8493553
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8239-43
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Determination of the distance between two spin labels attached to a macromolecule.
pubmed:affiliation
Department of Chemistry, Lawrence Berkeley Laboratory, University of California, Berkeley 94720, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't