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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1995-10-12
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pubmed:abstractText |
Establishment of loss-of-function phenotypes is often a key step in determining the biological function of a gene. We describe a procedure to obtain mutant petunia plants in which a specific gene with known sequence is inactivated by the transposable element dTph1. Leaves are collected from batches of 1000 plants with highly active dTph1 elements, pooled according to a three-dimensional matrix, and screened by PCR using a transposon- and a gene-specific primer. In this way individual plants with a dTph1 insertion can be identified by analysis of about 30 PCRs. We found insertion alleles for various genes at a frequency of about 1 in 1000 plants. The plant population can be preserved by selfing all the plants, so that it can be screened for insertions in many genes over a prolonged period.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-1318114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-1330322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-1423602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-1544919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-17746916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-1967052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-1968635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-2103441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-2152117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-2556711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-7910522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-7987417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-8100547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-8106081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-8130799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-8170935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-8269513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-8281187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-8380898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-8385803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667260-8395047
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
92
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pubmed:geneSymbol |
ap2a,
dTph1,
jaf13,
myb1,
myb27,
sha3,
sha4,
sha6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
8149-53
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:7667260-Alleles,
pubmed-meshheading:7667260-Base Sequence,
pubmed-meshheading:7667260-DNA Primers,
pubmed-meshheading:7667260-DNA Transposable Elements,
pubmed-meshheading:7667260-Gene Expression Regulation, Plant,
pubmed-meshheading:7667260-Genes, Plant,
pubmed-meshheading:7667260-Molecular Sequence Data,
pubmed-meshheading:7667260-Mutagenesis, Insertional,
pubmed-meshheading:7667260-Plants,
pubmed-meshheading:7667260-Polymerase Chain Reaction
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pubmed:year |
1995
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pubmed:articleTitle |
Targeted gene inactivation in petunia by PCR-based selection of transposon insertion mutants.
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pubmed:affiliation |
Department of Genetics, Vrije Universiteit, Amsterdam, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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