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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1995-10-12
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pubmed:abstractText |
We have previously described a series of human immunodeficiency virus type 1-based vectors in which efficient RNA encapsidation appeared to correlate with the presence of a 1.1-kb env gene fragment encompassing the Rev-responsive element (RRE). In this report, we explore in detail the role of the RRE and flanking env sequences in vector expression and RNA encapsidation. The analysis of a new series of vectors containing deletions within the env fragment failed to identify a discrete packaging signal, although the loss of certain sequences reduced packaging efficiency three- to fourfold. Complete removal of the env fragment resulted in a 100-fold decrease in the vector transduction titer but did not abolish RNA encapsidation. We conclude that the RRE and 3' env sequences are not essential for human immunodeficiency virus type 1 vector encapsidation but may be important in vectors in which a heterologous gene has been placed adjacent to the 5' packaging signal, potentially disrupting its structure.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-1560523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-1585635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-1727477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-1885765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-1985215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-2109098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-2194165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-2204725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-2410792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-2726755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-2760989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-3001360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-3012355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-3016552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-3094962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-3323813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666564-8510213
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:geneSymbol |
env
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6588-92
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7666564-Animals,
pubmed-meshheading:7666564-Base Sequence,
pubmed-meshheading:7666564-Capsid,
pubmed-meshheading:7666564-Cell Line,
pubmed-meshheading:7666564-Cercopithecus aethiops,
pubmed-meshheading:7666564-Genes, env,
pubmed-meshheading:7666564-Genetic Vectors,
pubmed-meshheading:7666564-HIV Long Terminal Repeat,
pubmed-meshheading:7666564-HIV-1,
pubmed-meshheading:7666564-HeLa Cells,
pubmed-meshheading:7666564-Humans,
pubmed-meshheading:7666564-Molecular Sequence Data,
pubmed-meshheading:7666564-Mutagenesis, Site-Directed,
pubmed-meshheading:7666564-Oligodeoxyribonucleotides,
pubmed-meshheading:7666564-RNA, Viral,
pubmed-meshheading:7666564-Restriction Mapping,
pubmed-meshheading:7666564-Sequence Deletion,
pubmed-meshheading:7666564-Transfection,
pubmed-meshheading:7666564-Virion
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pubmed:year |
1995
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pubmed:articleTitle |
cis-acting sequences involved in human immunodeficiency virus type 1 RNA packaging.
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pubmed:affiliation |
Department of Medicine, Addenbrooke's Hospital, Cambridge, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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